Activités-PGE

For bacterial cultures:

Incubators, centrifuges and spectrophotometers

- NewBrunswick incubators Innova I26 et I42 (*2): with shaking and cooling capability

- Eppendorf centrifuges 5910Ri ; 5430RF and 5415R

-Spectrophotometers for monitoring absorbance

For transformation of bacterial cells:

Electroporator (*2)

Cell transformation by electroporation (using an electric field to make cell membranes permeable)

For extractions of nucleic acids and proteins:

FastPrep and TissuLyser

• Designed to quickly and efficiently homogenize, grind, or lyse biological samples
• Sample volumes : ∼ 200 μL to 50 mL ; up to ∼ 25 g

Pour nucleic acid and proteins assays:

Nanodrop

• UV quantification of single- and double-stranded DNA and RNA
• Detection from very small volumes (0.3 µL)
• Also features a vortex mixer and absorbance reading (in cuvette, wavelength from 200 to 900 nm)

  

Qubit

• Quantification by fluorometric method of single- and double-stranded DNA, RNA, and proteins
  

  

For quality control of DNA, RNA, and protein samples:

Bioanalyser 2100 (Agilent)

• Microfluidics technique
• Ability to perform both electrophoresis separations and analyses of cell fluorescence parameters using flow cytometry

For nucleic acid amplifications:

PCR machines (Eppendorf)

• End-point PCR
• Possible gradient of 8 temperatures
• Use in strips or plates

  

qPCR CFX-Duet (BioRad)

• Real-time PCR
• Possible gradient of 8 temperatures
• Full plate fluorescence reading in 3 seconds for FAM/SybrGreen and 12 seconds for all channels
• Royalty-free software (Windows only)

For DNA sequencing:

Sequencer NextSeq1000 (Illumina)

• Metagenomic, metatranscriptomique, AmpliconSeq, RNASeq…
• FlowCell P1, P2 ; en 100, 200 (P2 only), 300 or 600pb
• Max run of 42h
• Up to 240Gb ouput
• Up to 400M in Single-End Reads

For membrane transfers and fluorescence quantification:

iBlot

• Rapid transfert of DNA, RNA or proteins onto membrane

• 

C-DiGit blot scanner

• Chemiluminescence Western blot detection

  

For Pulse-Field Gel Electrophoresis (PFGE):

CHEF-DR® II Pulsed Field Electrophoresis Systems

• Separation of DNA fragments > 20 kb
• Determining the size of a genome
• Determining the number of replicons contained in a genome.