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Home > Research Teams > Dynamique Microbienne et Transmission Virale

DMTV

Team Leader : Claire VALIENTE MORO
Coleader: Guillaume MINARD

Team:

Permanent members
LEGRAS-LACHUER Catherine Lecturer (MCF), UCB
LUIS Patricia Lecturer (MCF), UCB
MARTIN Edwige Assistant Ingénier (AI), CNRS
MINARD Guillaume Lecturer (MCF), UCB
POTIER Patrick Professor (PR), UCB
TRAN-VAN Van Engineer (IE), CNRS
VALIENTE-MORO Claire Lecturer (MCF), UCB
VALLON Laurent Technician, UCB
Personnels non permanents
LA POLLA Remi PhD student
RAQUIN Vincent Postdoctorant
Anciens membres
ACOSTA Erika PhD student, IPN Mexique
CARLINO Antoine Technician, CNRS
DEMICHEL Colin PhD student(2017-2018)
DOUAM Florian PhD student (2010-2013)
GONZALEZ Victor Chercheur invité UNAM Mexique
GUEGAN Morgane PhD student(2016-2019)
GRIZARD Stéphanie post-Doctoral fellow, UCB
LAVILLETTE Dimitri Researcher, CNRS
MAURIN Guillemette PhD student , ENS Lyon
MAVINGUI Patrick Research Director, CNRS
RAHARIMALALA Fara PhD student , UCB et Université Antananarivo
RANCÈS Edwige PhD student , UCB
RAQUIN Vincent PhD student , UCB
RENÉ-MARTELLET Magalie PhD student , UCB VetAgro Sup
SAUCEREAU Yoann PhD student (2013-2016)
TRAN Florence Technician, CNRS
VORONIN Denis Postdoctorant, CNRS
ZOUACHE Karima PhD student , UCB
ZOUACHE Karima post-Doctoral fellow, UCB

Infectious diseases are a global concern, since they remain the second leading cause of death worldwide, and induce annual loss of 40% of agricultural production. One of the major public health challenges is to provide methods to fight against the emergence and spreading of pathogenic infectious agents. Understanding which ecological factors are involved in the emergence may help to circumvent or at least reduce the infectious risks. We use as models the arboviruses (arthropod-borne viruses), including Chikungunya virus and Dengue virus, and the mosquito vector Aedes albopictus.

Until recently, the investigation of infectious diseases transmitted by vectors was essentially based on a vertical approach, bipartite, restricted to pathogen and its vector/reservoir or host. We are implementing a holistic approach that takes into account the different players (identity and density) and different levels (gene, molecular, cellular, individual and population) of interactions (Fig. 1). This systemic approach will help understanding at which levels of the pathogen-vector-symbiont-host interactions, anthropogenic factors and/or natural disturbance of the environment can lead to invasion and adaptation of vectors and generate the emergence and spreading of infectious agents.

Fig. 1 : The mosquito holobiont in vectorial pathosystem

To conduct our research, we combine field surveys and experimental studies. We recently constructed an level 2 insectarium at La Doua Campus and we contributed to the implementation of level 3 insectarium at Gerland Campus (UMS 3444, Bioscience).

Our integrated approach can enable the development of innovative methods
respectful of environment.

Theme 1. Ecology and evolution of vectors and their microbiota

In the light of the recent theory of the holobiont unit, we hypothesize that bacterial microbiota could contribute to the adaptive potential of mosquitoes. To that end, we explore what are the genomic, microbiological and ecological bases behind the highly invasive behaviour and ecological plasticity of Ae. albopictus. Thus, we undertake (i) the sampling of mosquito-field populations from various geographic origins and (ii)
the comparative analysis of host genetic populations and their bacterial metagenomic (metataxogenomic and metatrancriptomic). In parallel, we perform experimental infections of mosquitoes to study the mosquito-bacterial partnership when faced abiotic and biotic factors.

Fig. 2. Our study model: the tiger mosquito Aedes albopictus

Fig.3. Breeding of Ae. albopictus in our level 2 insectary

Theme 2. Multipartite interactions and vector transmission

Our studies and those of other international research groups have shown that some bacterial taxa may interfere with the vector competence of arthropods. The most studied bacterium in mosquitoes is the alphaproteobacteria Wolbachia. However, molecular and cellular mechanisms involved in multipartite interactions remain largely unknown. Using our cellular model (Fig. 4), we perform global functional analysis (transcriptomic and proteomic of interactions) of the mosquito responses to multi-
infections (Fig. 5). These approaches will allow identifying certain determinants that could be tested in insecta and some being potential candidates for the development of innovative control strategies.

Fig. 4. C636 cell line infected with Wolbachia (white arrows)

Fig. 5. Comparative analysis

Keywords: Biodiversity, adaptation, invasion, ecology and health, infectious diseases, bio-phylo-geography, host-pathogen interactions, arboviroses, virus transmission, alphavirus (Chikungunya), flavivirus (Dengue), microbiota (microbiome), Wolbachia, metagenomics (bacteriome, virome), (meta)transcriptomics (symbionts, hosts), experimental evolution, extended phenotype, holobiont (hologenome), vectors, mosquitoes, Aedes albopictus.

Bibliography :

2020



  • Gaudino, M., Aurine, N., Dumont, C., Fouret, J., Ferren, M., Mathieu, C., Reynard, O., Volchkov, V.E., Legras-Lachuer, C., Georges-Courbot, M.-C., Horvat, B., 2020. High Pathogenicity of Nipah Virus from <i>Pteropus lylei</i> Fruit Bats, Cambodia. Emerging Infectious Diseases 26, 104-113. https://doi.org/10.3201/eid2601.191284


  • Guégan, M., Tran Van, V., Martin, E., Minard, G., Tran, F.H., Fel, B., Hay, A.-E., Simon, L., Barakat, M., Potier, P., Haichar, F.Z., Valiente Moro, C., 2020. Who is eating fructose within the <i>Aedes albopictus</i> gut microbiota? Environmental Microbiology 1462-2920.14915. https://doi.org/10.1111/1462-2920.14915

2019



  • Luis, P., Vallon, L., Tran, F.H., Hugoni, M., Tran Van, V., Mavingui, P., Minard, G., Valiente Moro, C., 2019. Aedes albopictus mosquitoes host a locally structured mycobiota with evidence of reduced fungal diversity in invasive populations. Fungal Ecology 39, 257-266. https://doi.org/10.1016/j.funeco.2019.02.004


  • Mateo, M., Reynard, S., Carnec, X., Journeaux, A., Baillet, N., Schaeffer, J., Picard, C., Legras-Lachuer, C., Allan, R., Perthame, E., Hillion, K.-H., Pietrosemoli, N., Dillies, M.-A., Barrot, L., Vallve, A., Barron, S., Fellmann, L., Gaillard, J.-C., Armengaud, J., Carbonnelle, C., Raoul, H., Tangy, F., Baize, S., 2019. Vaccines inducing immunity to Lassa virus glycoprotein and nucleoprotein protect macaques after a single shot. Science Translational Medicine 11, eaaw3163. https://doi.org/10.1126/scitranslmed.aaw3163

  • Michalet, S., Minard, G., Chevalier, W., Meiffren, G., Saucereau, Y., Tran Van, V., Comte, G., Tran, F.H., Valiente Moro, C., 2019. Identification of human skin bacteria attractive to the Asian Tiger mosquito. Environmental Microbiology 21, 4662-4674. https://doi.org/10.1111/1462-2920.14793


  • Minard, G., Tikhonov, G., Ovaskainen, O., Saastamoinen, M., 2019. The microbiome of the Melitaea cinxia butterfly shows marked variation but is only little explained by the traits of the butterfly or its host plant. Environmental Microbiology 21, 4253-4269. https://doi.org/10.1111/1462-2920.14786


  • Nicolas de Lamballerie, C., Pizzorno, A., Dubois, J., Julien, T., Padey, B., Bouveret, M., Traversier, A., Legras-Lachuer, C., Lina, B., Boivin, G., Terrier, O., Rosa-Calatrava, M., 2019. Characterization of cellular transcriptomic signatures induced by different respiratory viruses in human reconstituted airway epithelia. Scientific Reports 9, 11493. https://doi.org/10.1038/s41598-019-48013-7


  • Pizzorno, A., Terrier, O., Nicolas de Lamballerie, C., Julien, T., Padey, B., Traversier, A., Roche, M., Hamelin, M.-E., Rhéaume, C., Croze, S., Escuret, V., Poissy, J., Lina, B., Legras-Lachuer, C., Textoris, J., Boivin, G., Rosa-Calatrava, M., 2019. Repurposing of Drugs as Novel Influenza Inhibitors From Clinical Gene Expression Infection Signatures. Frontiers in Immunology 10, 60. https://doi.org/10.3389/fimmu.2019.00060


  • Rosa, E., Minard, G., Lindholm, J., Saastamoinen, M., 2019. Moderate plant water stress improves larval development, and impacts immunity and gut microbiota of a specialist herbivore. PLOS ONE 14, e0204292. https://doi.org/10.1371/journal.pone.0204292


  • Theillet, G., Grard, G., Galla, M., Maisse, C., Enguehard, M., Cresson, M., Dalbon, P., Leparc‐Goffart, I.L., Bedin, F., 2019. Detection of chikungunya virus-specific IgM on laser-cut paper-based device using pseudo-particles as capture antigen. Journal of Medical Virology 91, 899-910. https://doi.org/10.1002/jmv.25420

2018



  • Boccoz, S.A., Fouret, J., Roche, M., Lachuer, J., Legras-Lachuer, C., Corgier, B.P., Marquette, C.A., 2018. Massively parallel and multiplex blood group genotyping using next-generation-sequencing. Clinical Biochemistry 60, 71-76. https://doi.org/10.1016/j.clinbiochem.2018.07.010


  • Douam, F., Fusil, F., Enguehard, M., Dib, L., Nadalin, F., Schwaller, L., Hrebikova, G., Mancip, J., Mailly, L., Montserret, R., Ding, Q., Maisse, C., Carlot, E., Xu, K., Verhoeyen, E., Baumert, T.F., Ploss, A., Carbone, A., Cosset, F.-L., Lavillette, D., 2018. A protein coevolution method uncovers critical features of the Hepatitis C Virus fusion mechanism. PLOS Pathogens 14, e1006908. https://doi.org/10.1371/journal.ppat.1006908


  • Guégan, M., Minard, G., Tran, F.H., Tran Van, V., Dubost, A., Valiente Moro, C., 2018. Short-term impacts of anthropogenic stressors on Aedes albopictus mosquito vector microbiota. FEMS Microbiology Ecology. https://doi.org/10.1093/femsec/fiy188


  • Guégan, M., Zouache, K., Démichel, C., Minard, G., Tran Van, V., Potier, P., Mavingui, P., Valiente Moro, C., 2018. The mosquito holobiont: fresh insight into mosquito-microbiota interactions. Microbiome 6, 49. https://doi.org/10.1186/s40168-018-0435-2


  • Tetreau, G., Grizard, S., Patil, C.D., Tran, F.-H., Tran Van, V., Stalinski, R., Laporte, F., Mavingui, P., Després, L., Valiente Moro, C., 2018. Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis. Parasites & Vectors 11. https://doi.org/10.1186/s13071-018-2741-8

2017



  • Dickson, L.B., Jiolle, D., Minard, G., Moltini-Conclois, I., Volant, S., Ghozlane, A., Bouchier, C., Ayala, D., Paupy, C., Moro, C.V., Lambrechts, L., 2017. Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector. Science Advances 3, e1700585. https://doi.org/10.1126/sciadv.1700585


  • Goubert, C., Henri, H., Minard, G., Valiente Moro, C., Mavingui, P., Vieira, C., Boulesteix, M., 2017. High-throughput sequencing of transposable element insertions suggests adaptive evolution of the invasive Asian tiger mosquito towards temperate environments. Molecular Ecology 26, 3968-3981. https://doi.org/10.1111/mec.14184


  • Minard, G., Tran Van, V., Tran, F.H., Melaun, C., Klimpel, S., Koch, L.K., Ly Huynh Kim, K., Huynh Thi Thuy, T., Tran Ngoc, H., Potier, P., Mavingui, P., Valiente Moro, C., 2017. Identification of sympatric cryptic species of Aedes albopictus subgroup in Vietnam: new perspectives in phylosymbiosis of insect vector. Parasites & Vectors 10, 276. https://doi.org/10.1186/s13071-017-2202-9


  • René-Martellet, M., Minard, G., Massot, R., Tran Van, V., Valiente Moro, C., Chabanne, L., Mavingui, P., 2017. Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona. Parasites & Vectors 10, 416. https://doi.org/10.1186/s13071-017-2352-9


  • Saucereau, Y., Valiente Moro, C., Dieryckx, C., Dupuy, J.-W., Tran, F.H., Girard, V., Potier, P., Mavingui, P., 2017. Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon. BMC Genomics 18, 635. https://doi.org/10.1186/s12864-017-3985-y


  • Wierinckx, A., Roche, M., Legras-Lachuer, C., Trouillas, J., Raverot, G., Lachuer, J., 2017. MicroRNAs in pituitary tumors. Molecular and Cellular Endocrinology, Small non-coding RNAs (and microRNAs) in endocrinology 456, 51-61. https://doi.org/10.1016/j.mce.2017.01.021


  • Zotzmann, S., Steinbrink, A., Schleich, K., Frantzmann, F., Xoumpholphakdy, C., Spaeth, M., Valiente Moro, C., Mavingui, P., Klimpel, S., 2017. Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany. Parasitology Research 116, 1899-1906. https://doi.org/10.1007/s00436-017-5466-2

2016



  • Debing, Y., Ramière, C., Dallmeier, K., Piorkowski, G., Trabaud, M.-A., Lebossé, F., Scholtès, C., Roche, M., Legras-Lachuer, C., de Lamballerie, X., André, P., Neyts, J., 2016. Hepatitis E virus mutations associated with ribavirin treatment failure result in altered viral fitness and ribavirin sensitivity. Journal of Hepatology. https://doi.org/10.1016/j.jhep.2016.05.002


  • Goubert, C., Minard, G., Vieira, C., Boulesteix, M., 2016. Population genetics of the Asian tiger mosquito Aedes albopictus, an invasive vector of human diseases. Heredity. https://doi.org/10.1038/hdy.2016.35


  • Moutailler, S., Valiente Moro, C., Vaumourin, E., Michelet, L., Tran, F.H., Devillers, E., Cosson, J.-F., Gasqui, P., Tran Van, V., Mavingui, P., Vourc’h, G., Vayssier-Taussat, M., 2016. Co-infection of Ticks: The Rule Rather Than the Exception. PLOS Neglected Tropical Diseases 10, e0004539. https://doi.org/10.1371/journal.pntd.0004539


  • Wilkinson, D.A., Duron, O., Cordonin, C., Gomard, Y., Ramasindrazana, B., Mavingui, P., Goodman, S.M., Tortosa, P., 2016. The bacteriome of bat flies (Nycteribiidae) from the Malagasy region: a community shaped by host ecology, bacterial transmission mode, and host-vector specificity. Applied and Environmental Microbiology 82, 1778-1788. https://doi.org/10.1128/AEM.03505-15