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Accueil > Equipes de Recherche > Dynamique Microbienne et Transmission Virale > DMTV

DMTV

Team Leader : Claire VALIENTE MORO

Team :

Permanent members
LAVILETTE Dimitri Research Scientist (CR), CNRS (HDR)
LEGRAS-LACHUER Catherine Lecturer (MCF), UCB
MAVINGUI Patrick Research Director (DR), CNRS
MINARD Guillaume Lecturer (MCF), UCB
POTIER Patrick Professor (PR), UCB
TRAN-VAN Van Engineer (IE), CNRS
VALIENTE-MORO Claire Lecturer (MCF), UCB
Personnels non permanents
DEMICHEL Colin PhD student (2017-2020)
ENGUELHARD Margot PhD student (2013-2016)
GUEGAN Morgane PhD student(2016-2019)
ZOUACHE Karima post-Doctoral fellow, UCB
Anciens membres
ACOSTA Erika PhD student, IPN Mexique
DOUAM Florian PhD student (2010-2013)
GONZALEZ Victor Chercheur invité UNAM Mexique
MAURIN Guillemette PhD student , ENS Lyon
RAHARIMALALA Fara PhD student , UCB et Université Antananarivo
RANCÈS Edwige PhD student , UCB
RAQUIN Vincent PhD student , UCB
RENÉ-MARTELLET Magalie PhD student , UCB VetAgro Sup
SAUCEREAU Yoann PhD student (2013-2016)
TRAN Florence Technician, CNRS
VORONIN Denis Postdoctorant, CNRS
ZOUACHE Karima PhD student , UCB

Infectious diseases are a global concern, since they remain the second leading cause of death worldwide, and induce annual loss of 40% of agricultural production. One of the major public health challenges is to provide methods to fight against the emergence and spreading of pathogenic infectious agents. Understanding which ecological factors are involved in the emergence may help to circumvent or at least reduce the infectious risks. We use as models the arboviruses (arthropod-borne viruses), including Chikungunya virus and Dengue virus, and the mosquito vector Aedes albopictus.

Until recently, the investigation of infectious diseases transmitted by vectors was essentially based on a vertical approach, bipartite, restricted to pathogen and its vector/reservoir or host. We are implementing a holistic approach that takes into account the different players (identity and density) and different levels (gene, molecular, cellular, individual and population) of interactions (Fig. 1). This systemic approach will help understanding at which levels of the pathogen-vector-symbiont-host interactions, anthropogenic factors and/or natural disturbance of the environment can lead to invasion and adaptation of vectors and generate the emergence and spreading of infectious agents.

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Fig. 1 : The mosquito holobiont in vectorial pathosystem

To conduct our research, we combine field surveys and experimental studies. We recently constructed an level 2 insectarium at La Doua Campus and we contributed to the implementation of level 3 insectarium at Gerland Campus (UMS 3444, Bioscience).

Our integrated approach can enable the development of innovative methods
respectful of environment.

Theme 1. Ecology and evolution of vectors and their microbiota

In the light of the recent theory of the holobiont unit, we hypothesize that bacterial microbiota could contribute to the adaptive potential of mosquitoes. To that end, we explore what are the genomic, microbiological and ecological bases behind the highly invasive behaviour and ecological plasticity of Ae. albopictus. Thus, we undertake (i) the sampling of mosquito-field populations from various geographic origins and (ii)
the comparative analysis of host genetic populations and their bacterial metagenomic (metataxogenomic and metatrancriptomic). In parallel, we perform experimental infections of mosquitoes to study the mosquito-bacterial partnership when faced abiotic and biotic factors.

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Fig. 2. Our study model : the tiger mosquito Aedes albopictus

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Fig.3. Breeding of Ae. albopictus in our level 2 insectary

Theme 2. Multipartite interactions and vector transmission

Our studies and those of other international research groups have shown that some bacterial taxa may interfere with the vector competence of arthropods. The most studied bacterium in mosquitoes is the alphaproteobacteria Wolbachia. However, molecular and cellular mechanisms involved in multipartite interactions remain largely unknown. Using our cellular model (Fig. 4), we perform global functional analysis (transcriptomic and proteomic of interactions) of the mosquito responses to multi-
infections (Fig. 5). These approaches will allow identifying certain determinants that could be tested in insecta and some being potential candidates for the development of innovative control strategies.

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Fig. 4. C636 cell line infected with Wolbachia (white arrows)

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Fig. 5. Comparative analysis

Keywords : Biodiversity, adaptation, invasion, ecology and health, infectious diseases, bio-phylo-geography, host-pathogen interactions, arboviroses, virus transmission, alphavirus (Chikungunya), flavivirus (Dengue), microbiota (microbiome), Wolbachia, metagenomics (bacteriome, virome), (meta)transcriptomics (symbionts, hosts), experimental evolution, extended phenotype, holobiont (hologenome), vectors, mosquitoes, Aedes albopictus.

Bibliography :

2018



  • Douam, F., Fusil, F., Enguehard, M., Dib, L., Nadalin, F., Schwaller, L., Hrebikova, G., Mancip, J., Mailly, L., Montserret, R., Ding, Q., Maisse, C., Carlot, E., Xu, K., Verhoeyen, E., Baumert, T.F., Ploss, A., Carbone, A., Cosset, F.-L., Lavillette, D., 2018. A protein coevolution method uncovers critical features of the Hepatitis C Virus fusion mechanism. PLOS Pathogens 14, e1006908. https://doi.org/10.1371/journal.ppat.1006908


  • Guégan, M., Zouache, K., Démichel, C., Minard, G., Tran Van, V., Potier, P., Mavingui, P., Valiente Moro, C., 2018. The mosquito holobiont: fresh insight into mosquito-microbiota interactions. Microbiome 6, 49. https://doi.org/10.1186/s40168-018-0435-2


  • Tetreau, G., Grizard, S., Patil, C.D., Tran, F.-H., Tran Van, V., Stalinski, R., Laporte, F., Mavingui, P., Després, L., Valiente Moro, C., 2018. Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis. Parasites & Vectors 11. https://doi.org/10.1186/s13071-018-2741-8

2017



  • Dickson, L.B., Jiolle, D., Minard, G., Moltini-Conclois, I., Volant, S., Ghozlane, A., Bouchier, C., Ayala, D., Paupy, C., Moro, C.V., Lambrechts, L., 2017. Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector. Science Advances 3, e1700585. https://doi.org/10.1126/sciadv.1700585


  • Goubert, C., Henri, H., Minard, G., Valiente Moro, C., Mavingui, P., Vieira, C., Boulesteix, M., 2017. High-throughput sequencing of transposable element insertions suggests adaptive evolution of the invasive Asian tiger mosquito towards temperate environments. Molecular Ecology 26, 3968-3981. https://doi.org/10.1111/mec.14184


  • Minard, G., Tran Van, V., Tran, F.H., Melaun, C., Klimpel, S., Koch, L.K., Ly Huynh Kim, K., Huynh Thi Thuy, T., Tran Ngoc, H., Potier, P., Mavingui, P., Valiente Moro, C., 2017. Identification of sympatric cryptic species of Aedes albopictus subgroup in Vietnam: new perspectives in phylosymbiosis of insect vector. Parasites & Vectors 10, 276. https://doi.org/10.1186/s13071-017-2202-9


  • René-Martellet, M., Minard, G., Massot, R., Tran Van, V., Valiente Moro, C., Chabanne, L., Mavingui, P., 2017. Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona. Parasites & Vectors 10, 416. https://doi.org/10.1186/s13071-017-2352-9


  • Saucereau, Y., Valiente Moro, C., Dieryckx, C., Dupuy, J.-W., Tran, F.H., Girard, V., Potier, P., Mavingui, P., 2017. Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon. BMC Genomics 18, 635. https://doi.org/10.1186/s12864-017-3985-y


  • Wierinckx, A., Roche, M., Legras-Lachuer, C., Trouillas, J., Raverot, G., Lachuer, J., 2017. MicroRNAs in pituitary tumors. Molecular and Cellular Endocrinology, Small non-coding RNAs (and microRNAs) in endocrinology 456, 51-61. https://doi.org/10.1016/j.mce.2017.01.021


  • Zotzmann, S., Steinbrink, A., Schleich, K., Frantzmann, F., Xoumpholphakdy, C., Spaeth, M., Valiente Moro, C., Mavingui, P., Klimpel, S., 2017. Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany. Parasitology Research 116, 1899-1906. https://doi.org/10.1007/s00436-017-5466-2

2016



  • Debing, Y., Ramière, C., Dallmeier, K., Piorkowski, G., Trabaud, M.-A., Lebossé, F., Scholtès, C., Roche, M., Legras-Lachuer, C., de Lamballerie, X., André, P., Neyts, J., 2016. Hepatitis E virus mutations associated with ribavirin treatment failure result in altered viral fitness and ribavirin sensitivity. Journal of Hepatology. https://doi.org/10.1016/j.jhep.2016.05.002


  • Goubert, C., Minard, G., Vieira, C., Boulesteix, M., 2016. Population genetics of the Asian tiger mosquito Aedes albopictus, an invasive vector of human diseases. Heredity. https://doi.org/10.1038/hdy.2016.35


  • Moutailler, S., Valiente Moro, C., Vaumourin, E., Michelet, L., Tran, F.H., Devillers, E., Cosson, J.-F., Gasqui, P., Tran Van, V., Mavingui, P., Vourc’h, G., Vayssier-Taussat, M., 2016. Co-infection of Ticks: The Rule Rather Than the Exception. PLOS Neglected Tropical Diseases 10, e0004539. https://doi.org/10.1371/journal.pntd.0004539


  • Wilkinson, D.A., Duron, O., Cordonin, C., Gomard, Y., Ramasindrazana, B., Mavingui, P., Goodman, S.M., Tortosa, P., 2016. The bacteriome of bat flies (Nycteribiidae) from the Malagasy region: a community shaped by host ecology, bacterial transmission mode, and host-vector specificity. Applied and Environmental Microbiology 82, 1778-1788. https://doi.org/10.1128/AEM.03505-15

2015



  • Calattini, S., Fusil, F., Mancip, J., Dao Thi, V.L., Granier, C., Gadot, N., Scoazec, J.-Y., Zeisel, M., Baumert, T.F., Lavillette, D., Dreux, M., Cosset, F.-L., 2015. Functional and Biochemical Characterization of HCV Particles Produced in a Humanized Liver Mouse Model. Journal of Biological Chemistry jbc.M115.662999. https://doi.org/10.1074/jbc.M115.662999


  • Douam, F., Bobay, L.M., Maurin, G., Fresquet, J., Calland, N., Maisse, C., Durand, T., Cosset, F.L., Féray, C., Lavillette, D., 2015. Specialization of hepatitis C virus envelope glycoproteins for B-lymphocytes in chronically infected patients. Journal of Virology JVI.02516-15. https://doi.org/10.1128/JVI.02516-15

  • Douam, F., Lavillette, D., Cosset, F.-L., 2015. The mechanism of HCV entry into host cells. Progress in Molecular Biology and Translational Science 129, 63-107. https://doi.org/10.1016/bs.pmbts.2014.10.003

  • Faure, D., Bonin, P., Duran, R., Microbial Ecology EC2CO consortium, 2015. Environmental microbiology as a mosaic of explored ecosystems and issues. Environmental Science and Pollution Research International 22, 13577-13598. https://doi.org/10.1007/s11356-015-5164-5


  • Fénéant, L., Potel, J., François, C., Sané, F., Douam, F., Belouzard, S., Calland, N., Vausselin, T., Rouillé, Y., Descamps, V., Baumert, T.F., Duverlie, G., Lavillette, D., Hober, D., Dubuisson, J., Wychowski, C., Cocquerel, L., 2015. New insights into the understanding of hepatitis C virus entry and cell-to-cell transmission by using the ionophore Monensin A. Journal of Virology JVI.00192-15. https://doi.org/10.1128/JVI.00192-15


  • George, D.R., Finn, R.D., Graham, K.M., Mul, M.F., Maurer, V., Valiente Moro, C., Sparagano, O.A.E., 2015. Should the poultry red mite Dermanyssus gallinae be of wider concern for veterinary and medical science? Parasites & Vectors 8, 1-10. https://doi.org/10.1186/s13071-015-0768-7


  • Goubert, C., Modolo, L., Vieira, C., Valiente Moro, C., Mavingui, P., Boulesteix, M., 2015. De novo assembly and annotation of the Asian tiger mosquito (Aedes albopictus) repeatome with dnaPipeTE from raw genomic reads and comparative analysis with the yellow fever mosquito (Aedes aegypti). Genome Biology and Evolution. https://doi.org/10.1093/gbe/evv050


  • Marnata, C., Saulnier, A., Mompelat, D., Krey, T., Cohen, L., Boukadida, C., Warter, L., Fresquet, J., Vasiliauskaite, I., Escriou, N., Cosset, F.-L., Rey, F.A., Lanford, R.E., Karayiannis, P., Rose, N.J., Lavillette, D., Martin, A., 2015. Determinants involved in hepatitis C virus and GB virus B primate host restriction. Journal of Virology JVI.01161-15. https://doi.org/10.1128/JVI.01161-15


  • Minard, G., Tran, F.H., Tran Van, V., Goubert, C., Bellet, C., Lambert, G., Khanh, H.K.L., Huynh, T., Mavingui, P., Valiente Moro, C., 2015. French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives. Microbial Symbioses 6, 970. https://doi.org/10.3389/fmicb.2015.00970


  • Murphy, L., Varela, M., Desloire, S., Ftaich, N., Murgia, C., Golder, M., Neil, S., Spencer, T.E., Wootton, S.K., Lavillette, D., Terzian, C., Palmarini, M., Arnaud, F., 2015. The Sheep Tetherin Paralog oBST2B Blocks Envelope Glycoprotein Incorporation into Nascent Retroviral Virions. Journal of Virology 89, 535-544. https://doi.org/10.1128/JVI.02751-14


  • Raquin, V., Valiente Moro, C., Saucereau, Y., Tran, F.H., Potier, P., Mavingui, P., 2015. Native Wolbachia from Aedes albopictus Blocks Chikungunya Virus Infection In Cellulo. PLOS ONE 10, e0125066. https://doi.org/10.1371/journal.pone.0125066


  • René-Martellet, M., Valiente Moro, C., Chêne, J., Bourdoiseau, G., Chabanne, L., Mavingui, P., 2015. Update on epidemiology of canine babesiosis in Southern France. BMC Veterinary Research 11. https://doi.org/10.1186/s12917-015-0525-3


  • Roche, M., Wierinckx, A., Croze, S., Rey, C., Legras-Lachuer, C., Morel, A.-P., Fusco, A., Raverot, G., Trouillas, J., Lachuer, J., 2015. Deregulation of miR-183 and KIAA0101 in Aggressive and Malignant Pituitary Tumors. Frontiers in Medicine 2. https://doi.org/10.3389/fmed.2015.00054

  • Wisniewski-Dyé, F., Drogue, B., Borland, S., Prigent-Combaret, C., Borziak, K., Zhulin, I.B., Lozano, L., Gonzáles, V., Mavingui, P., 2015. Core and Accessory Genomes of the Diazotroph Azospirillum, dans: Biological Nitrogen Fixation, 2 Volume Set. p. 253.

2014



  • Bocchetta, S., Maillard, P., Yamamoto, M., Gondeau, C., Douam, F., Lebreton, S., Lagaye, S., Pol, S., Helle, F., Plengpanich, W., Guérin, M., Bourgine, M., Michel, M.L., Lavillette, D., Roingeard, P., le Goff, W., Budkowska, A., 2014. Up-Regulation of the ATP-Binding Cassette Transporter A1 Inhibits Hepatitis C Virus Infection. PLoS ONE 9, e92140. https://doi.org/10.1371/journal.pone.0092140


  • Cartier, F., Marcq, I., Douam, F., Ossart, C., Regnier, A., Debuysscher, V., Lavillette, D., Bouhlal, H., 2014. The Expression of the Hepatocyte SLAMF3 (CD229) Receptor Enhances the Hepatitis C Virus Infection. PLOS ONE 9, e99601. https://doi.org/10.1371/journal.pone.0099601


  • Douam, F., Dao Thi, V.L., Maurin, G., Fresquet, J., Mompelat, D., Zeisel, M.B., Baumert, T.F., Cosset, F.-L., Lavillette, D., 2014. Critical interaction between E1 and E2 glycoproteins determines binding and fusion properties of hepatitis C virus during cell entry. Hepatology n/a–n/a. https://doi.org/10.1002/hep.26733


  • Dupinay, T., Pounder, K.C., Ayral, F., Laaberki, M.-H., Marston, D.A., Lacôte, S., Rey, C., Barbet, F., Voller, K., Nazaret, N., Artois, M., Marianneau, P., Lachuer, J., Fooks, A.R., Pépin, M., Legras-Lachuer, C., McElhinney, L.M., 2014. Detection and genetic characterization of Seoul Virus from commensal brown rats in France. Virology Journal 11, 32. https://doi.org/10.1186/1743-422X-11-32


  • Girard-Gagnepain, A., Amirache, F., Costa, C., Lévy, C., Frecha, C., Fusil, F., Nègre, D., Lavillette, D., Cosset, F.-L., Verhoeyen, E., 2014. Baboon envelope pseudotyped lentiviral vectors outperform VSV-G pseudotyped lentiviral vectors for gene transfer into cytokine-stimulated and resting hematopoietic stem cells. Blood. https://doi.org/10.1182/blood-2014-02-558163

  • Minard, G., 2014. Écologie du microbiote bactérien associé au moustique tigre Aedes albopictus : une approche "omique" pour l'exploration de l'holobionte vecteur (phdthesis). Université Claude Bernard - Lyon I.


  • Minard, G., Tran, F.H., Dubost, A., Tran Van, V., Mavingui, P., Valiente Moro, C., 2014. Pyrosequencing 16S rRNA genes of bacteria associated with wild tiger mosquito Aedes albopictus: a pilot study. Frontiers in Cellular and Infection Microbiology 4. https://doi.org/10.3389/fcimb.2014.00059


  • Vernin, C., Thenoz, M., Pinatel, C., Gessain, A., Gout, O., Delfau-Larue, M.-H., Nazaret, N., Legras-Lachuer, C., Wattel, E., Mortreux, F., 2014. HTLV-1 bZIP factor HBZ promotes cell proliferation and genetic instability by activating oncomiRs. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-13-3564