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Accueil > Pages Perso > Claire Valiente Moro

Equipe de Recherche Dynamique microbienne et transmission virale

Claire Valiente Moro

Maître de conférences


  • Thèmes de recherches :
    1. Rôle du microbiote bactérien dans l’adaptation des moustiques vecteurs
    2. Interactions multipartites (pathogènes, symbiotes, vecteurs) au sein des pathosystèmes vectoriels en prenant comme modèles d’étude les moustiques vecteurs d’arbovirus.

Parcours scientifique

  • MCF depuis Septembre 2009, UCB Lyon 1, Ecologie Microbienne
  • Post-doctorat (2007-2009), Université Blaise Pascal, LMGE, Clermont-Ferrand
  • Thèse en Ecologie Microbienne (2007). UCB Lyon 1


  • Co-encadrante de la thèse de Yoann Saucereau. Depuis 2013. "Immunité et symbiose chez le moustique tigre Aedes albopictus : implication dans la transmission des arbovirus"
  • Directrice de la thèse de Guillaume MINARD. 2011-2014. "Métagénomique fonctionnelle du microbiote et rôle dansl’adaptation et les capacités invasives du moustique Aedes albopictus"
  • Co-directrice de la thèse de Vincent RAQUIN. 2009-2012. « "Immunité innée et multi-infection microbienne chez le moustique : conséquences épidémiologiques des interactions multipartites dans la transmission des arbovirus"
  • Co-encadrante de la thèse de Magali RENE. 2010-2013. "Étude du rôle vecteur de Rhipicephalus sanguineus s.l. dans la transmission des babésioses canines en France : prévalence parasitaire, diversité génétique des vecteurs et épidémiologie"
  • Co-Responsable de la Plateforme d’Enseignement Pratique de Microbiologie du Département de Biologie de l’Université Claude Bernard Lyon1



  • Goubert C, et al. 2016. High-Throughput Sequencing of Transposable Elements Insertions Provides Evidence for Adaptive Evolution of the Invasive Asian Tiger Mosquito Towards Temperate Environments. bioRxiv. doi: 10.1101/049197.
    Résumé : Invasive species represent unique opportunities to evaluate the role of local adaptation during colonization of new environments. Among these, the Asian tiger mosquito, Aedes albopictus, is a threatening vector of several human viral diseases, including dengue, chikungunya and the emerging Zika fevers. Its broad presence in both temperate and tropical environments has sometimes been considered as the reflect of a great "ecological plasticity". However, no study has been conducted to assess the role of adaptive evolution in the ecological success of Ae. albopictus at the molecular level. In the present study we performed a genomic scan to search for potential signatures of selection leading to local adaptation in a hundred of field collected mosquitoes from native populations of Vietnam and temperate invasive populations of Europe. High throughput genotyping of transposable element insertions generated more than 120 000 polymorphic loci, which in their great majority revealed a virtual absence of structure between bio-geographic areas. Nevertheless, 92 outlier loci show a high level of differentiation between temperate and tropical populations. The majority of these loci segregates at high insertion frequencies among European populations, indicating that this pattern could have been caused by recent events of adaptive evolution in temperate areas. Six outliers were located near putative diapause effector genes, suggesting fine tunning of this critical pathway during local adaptation.
    Mots-clés : #7.


  • George DR, et al. 2015. Should the poultry red mite Dermanyssus gallinae be of wider concern for veterinary and medical science? Parasites & Vectors. 8:1-10. doi: 10.1186/s13071-015-0768-7.
    Résumé : The poultry red mite Dermanyssus gallinae is best known as a threat to the laying-hen industry; adversely affecting production and hen health and welfare throughout the globe, both directly and through its role as a disease vector. Nevertheless, D. gallinae is being increasingly implemented in dermatological complaints in non-avian hosts, suggesting that its significance may extend beyond poultry. The main objective of the current work was to review the potential of D. gallinae as a wider veterinary and medical threat. Results demonstrated that, as an avian mite, D. gallinae is unsurprisingly an occasional pest of pet birds. However, research also supports that these mites will feed from a range of other animals including: cats, dogs, rodents, rabbits, horses and man. We conclude that although reported cases of D. gallinae infesting mammals are relatively rare, when coupled with the reported genetic plasticity of this species and evidence of permanent infestations on non-avian hosts, potential for host-expansion may exist. The impact of, and mechanisms and risk factors for such expansion are discussed, and suggestions for further work made. Given the potential severity of any level of host-expansion in D. gallinae, we conclude that further research should be urgently conducted to confirm the full extent of the threat posed by D. gallinae to (non-avian) veterinary and medical sectors.
    Mots-clés : Avian mite dermatitis, Dermanyssus gallinae, Entomology, Gamasoidosis, Host expansion, Infectious Diseases, Non-host feeding, Parasitology, Tropical Medicine.

  • Goubert C, et al. 2015. De novo assembly and annotation of the Asian tiger mosquito (Aedes albopictus) repeatome with dnaPipeTE from raw genomic reads and comparative analysis with the yellow fever mosquito (Aedes aegypti). Genome Biology and Evolution. doi: 10.1093/gbe/evv050.
    Mots-clés : #7.

  • Minard G, et al. 2015. French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives. Microbial Symbioses. 6:970. doi: 10.3389/fmicb.2015.00970.
    Résumé : The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the 21st century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.
    Mots-clés : #7, Aedes albopictus, Dysgonomonas, holobiont, microbiota, microsatellite, Phylogeography, Wolbachia.

  • Raquin V, et al. 2015. Native Wolbachia from Aedes albopictus Blocks Chikungunya Virus Infection In Cellulo Bourtzis, K. PLOS ONE. 10:e0125066. doi: 10.1371/journal.pone.0125066.
    Mots-clés : #7.


  • Minard G, et al. 2014. Pyrosequencing 16S rRNA genes of bacteria associated with wild tiger mosquito Aedes albopictus: a pilot study. Frontiers in Cellular and Infection Microbiology. 4. doi: 10.3389/fcimb.2014.00059.
    Résumé : The Asian tiger mosquito Aedes (Stegomya) albopictus is an invasive species that has spread across the world in the last two decades, showing a great capacity to adapt to contrasting climates and environments. While demonstrated in many insects, the contribution of bacterial symbionts in Aedes ecology is a challenging aspect that needs to be investigated. Also some bacterial species have already been identified in Ae. albopictus using classical methods, but a more accurate survey of mosquito-associated bacterial diversity is needed to decipher the potential biological functions of bacterial symbionts in mediating or constraining insect adaptation. We surveyed the bacteria associated with field populations of Ae. albopictus from Madagascar by pyrosequencing 16S rRNA gene amplicons. Different aspects of amplicon preparation and sequencing depth were tested to optimize the breadth of bacterial diversity identified. The results revealed that all mosquitoes collected from different sites have a bacterial microbiota dominated by a single taxon, Wolbachia pipientis, which accounted for about 99% of all 92,615 sequences obtained. As Ae. albopictus is known to harbor two Wolbachia strains (wAlbA and wAlbB), a quantitative PCR was used to estimate the relative densities, (i.e., the bacteria-to-host gene ratios) of each strains in individual mosquitoes. Relative densities were between 6.25 × 100.01 and 5.47 × 100.1 for wAlbA and between 2.03 × 100.1 and 1.4 × 101 for wAlbB. Apart from Wolbachia, a total of 31 bacterial taxa were identified at the genus level using different method variations. Diversity index values were low and probably underestimated the true diversity due to the high abundance of Wolbachia sequences vastly outnumbering sequences from other taxa. Further studies should implement alternative strategies to specifically discard from analysis any sequences from Wolbachia, the dominant endosymbiotic bacterium in Ae. albopictus from this area.
    Mots-clés : #7, #ibio.


  • Minard G, Mavingui P, Valiente-Moro C. 2013. Diversity and function of bacterial microbiota in the mosquito holobiont. Parasites & Vectors. 6:146. doi: 10.1186/1756-3305-6-146.
    Résumé : Mosquitoes (Diptera: Culicidae) have been shown to host diverse bacterial communities that vary depending on the sex of the mosquito, the developmental stage, and ecological factors. Some studies have suggested a potential role of microbiota in the nutritional, developmental and reproductive biology of mosquitoes. Here, we present a review of the diversity and functions of mosquito-associated bacteria across multiple variation factors, emphasizing recent findings. Mosquito microbiota is considered in the context of possible extended phenotypes conferred on the insect hosts that allow niche diversification and rapid adaptive evolution in other insects. These kinds of observations have prompted the recent development of new mosquito control methods based on the use of symbiotically-modified mosquitoes to interfere with pathogen transmission or reduce the host life span and reproduction. New opportunities for exploiting bacterial function for vector control are highlighted.
    Mots-clés : #7, bacterial community, Extended phenotype, Microbiome, Mosquito, Symbiont.

  • Minard G, et al. 2013. Prevalence, genomic and metabolic profiles of <i>Acinetobacter</i> and <i>Asaia</i> associated with field-caught <i>Aedes albopictus</i> from Madagascar. FEMS Microbiology Ecology.
    Mots-clés : #7.

  • Valiente-Moro C, Tran FH, Raharimalala FN, Ravelonandro P, Mavingui P. 2013. Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus. BMC Microbiology. 13:70. doi: 10.1186/1471-2180-13-70.
    Résumé : The microbiota has been shown to play an important role in the biology of insects. In recent decades, significant efforts have been made to better understand the diversity of symbiotic bacteria associated with mosquitoes and assess their influence on pathogen transmission. Here, we report the bacterial composition found in field-caught Aedes albopictus populations by using culture-dependent methods.
    Mots-clés : #7.


  • Mavingui P, et al. 2012. Whole-Genome Sequence of <i>Wolbachia</i> Strain wAlbB, an Endosymbiont of Tiger Mosquito Vector <i>Aedes albopictus</i>. Journal of Bacteriology. 194:1840-1840. doi: 10.1128/JB.00036-12.
    Résumé : Although bacteria of the genus Wolbachia induced significant extended phenotypes to infected hosts, most molecular mechanisms involved are still unknown. To gain insight into the bacterial genetic determinants, we sequenced the whole genome of Wolbachia wAlbB strain, a commensal obligate intracellular of the tiger mosquito Aedes albopictus.
    Mots-clés : #3, #7.

  • Raharimalala F, et al. 2012. Biogeography of the two major arbovirus mosquito vectors, Aedes aegypti and Aedes albopictus (Diptera, Culicidae), in Madagascar. Parasites & Vectors. 5:56. doi: 10.1186/1756-3305-5-56.
    Résumé : In the past ten years, the Indian Ocean region has been the theatre of severe epidemics of chikungunya and dengue. These outbreaks coincided with a high increase in populations of Aedes albopictus that outcompete its sister taxon Aedes aegypti in most islands sampled. The objective of this work was to update the entomological survey of the two Aedes species in the island of Madagascar which has to face these arboviroses.
    Mots-clés : #7.

  • Raquin V, et al. 2012. Detection of dengue group viruses by fluorescence in situ hybridization. Parasites & vectors. 5:243. doi: 10.1186/1756-3305-5-243.
    Résumé : ABSTRACT: BACKGROUND: Dengue fever (DF) and dengue hemorrhagic fever (DHF) represent a global challenge in public health. It is estimated that 50 to 100 million infections occur each year causing approximately 20,000 deaths that are usually linked to severe cases like DHF and dengue shock syndrome. The causative agent of DF is dengue virus (genus Flavivirus) that comprises four distinct serotypes (DENV-1 to DENV-4). Fluorescence in situ hybridization (FISH) has been used successfully to detect pathogenic agents, but has not been implemented in detecting DENV. To improve our understanding of DENV infection and dissemination in host tissues, we designed specific probes to detect DENV in FISH assays. METHODS: Oligonucleotide probes were designed to hybridize with RNA from the broadest range of DENV isolates belonging to the four serotypes, but not to the closest Flavivirus genomes. Three probes that fit the criteria defined for FISH experiments were selected, targeting both coding and non-coding regions of the DENV genome. These probes were tested in FISH assays against the dengue vector Aedes albopictus (Diptera: Culicidae). The FISH experiments were led in vitro using the C6/36 cell line, and in vivo against dissected salivary glands, with epifluorescence and confocal microscopy. RESULTS: The three 60-nt oligonucleotides probes DENV-Probe A, B and C cover a broad range of DENV isolates from the four serotypes. When the three probes were used together, specific fluorescent signals were observed in C6/36 infected with each DENV serotypes. No signal was detected in either cells infected with close Flavivirus members West Nile virus or yellow fever virus. The same protocol was used on salivary glands of Ae. albopictus fed with a DENV-2 infectious blood-meal which showed positive signals in the lateral lobes of infected samples, with no significant signal in uninfected mosquitoes. CONCLUSION: Based on the FISH technique, we propose a way to design and use oligonucleotide probes to detect arboviruses. Results showed that this method was successfully implemented to specifically detect DENV in a mosquito cell line, as well as in mosquito salivary glands for the DENV-2 serotype. In addition, we emphasize that FISH could be an alternative method to detect arboviruses in host tissues, also offering to circumvent the discontinuity of antibodies used in immunofluorescent assays.
    Mots-clés : #7.

  • René M, et al. 2012. First evidence and molecular characterization of<i> Babesia vogeli</i> in naturally infected dogs and<i> Rhipicephalus sanguineus</i> ticks in southern France. Veterinary parasitology.
    Mots-clés : #7.

  • Valiente-Moro C, Bricheux G, Portelli C, Bohatier J. 2012. Comparative effects of the herbicides chlortoluron and mesotrione on freshwater microalgae. Environmental toxicology and chemistry / SETAC. 31:778-786. doi: 10.1002/etc.1749.
    Résumé : Extensive use of herbicides in agriculture is accompanied by the risk of environmental contamination of aquatic ecosystems. The present study shows the effects of the herbicides chlortoluron and mesotrione on three microalgae species: two chlorophyceae (Pediastrum tetras, Ankistrodesmus fusiformis) and one diatom (Amphora coffeaeformis). The authors calculated the IC50 for one chlorophyceae and the diatom. The order of toxicity (median inhibitory concentration [IC50]) for mesotrione was A. coffeaeformis (13.1 mg/L) > A. fusiformis (56.1 mg/L) and A. fusiformis (0.05 mg/L) > A. coffeaeformis (0.08 mg/L) for chlortoluron. The impact of herbicides applied at 0.2 mg/L was then examined in Erlenmeyer flasks by monitoring for growth, pigment content, and metabolic activity. Algal responses varied widely according to species and herbicide. For example, chlortoluron showed a significant inhibitory effect on the growth of A. coffeaeformis, whereas mesotrione induced an increase in cellular density in A. fusiformis. Other cellular parameters, such as pigment content in P. tetras, were stimulated by both herbicides. The results obtained confirmed that microalgae cultures are clearly affected by acute and chronic exposition to herbicides. Further monitoring should be carried out in the field to assess the impact of sublethal levels of toxicity and the growth-enhancing effects of mesotrione and chlortoluron on natural algae communities.
    Mots-clés : #7, Agriculture, Chlorophyta, Cyclohexanones, Diatoms, Fresh Water, Herbicides, Inhibitory Concentration 50, Microalgae, Phenylurea Compounds, Pigments, Biological, Toxicity Tests, Water Pollutants, Chemical.


  • Valiente-Moro C, Thioulouse J, Chauve C, Zenner L. 2011. Diversity, Geographic Distribution, and Habitat-Specific Variations of Microbiota in Natural Populations of the Chicken Mite, Dermanyssus gallinae. Journal of Medical Entomology. 48:788-796. doi: 10.1603/ME10113.
    Mots-clés : #7.


  • Thioulouse J, Valiente-Moro C. 2010. Online Reproducible Research: An Application to Multivariate Analysis of Bacterial DNA Fingerprint Data. The R Journal. 2:44–52.
    Mots-clés : #7.


  • De Luna CJ, Valiente-Moro C, Guy JH, Zenner L, Sparagano OAE. 2009. Endosymbiotic bacteria living inside the poultry red mite (Dermanyssus gallinae). Experimental & applied acarology. 48:105-113. doi: 10.1007/s10493-008-9230-2.
    Résumé : This study investigated the endosymbiotic bacteria living inside the poultry red mite collected from five samples of one commercial farm from the UK and 16 farms from France using genus-specific PCR, PCR-TTGE and DNA sequencing. Endosymbiotic bacteria are intracellular obligate organisms that can cause several phenotypic and reproductive anomalies to their host and they are found widespread living inside arthropods. The farm sampled from the UK was positive for bacteria of the genera Cardinium sp. and Spiroplasma sp. From France, 7 farms were positive for Cardinium sp., 1 farm was positive for Spiroplasma sp., 1 farm was positive for Rickettsiella sp. and 2 farms were positive for Schineria sp. However, it was not possible to detect the presence of the genus Wolbachia sp. which has been observed in other ectoparasites. This study is the first report of the presence of endosymbionts living inside the poultry red mite. The results obtained suggest that it may be possible that these bacterial endosymbionts cause biological modifications to the poultry red mite.
    Mots-clés : #7, Animals, Bacteroidetes, Base Sequence, Coxiellaceae, France, Great Britain, Mites, Molecular Sequence Data, Polymerase Chain Reaction, Spiroplasma, symbiosis, Xanthomonadaceae.

  • Valiente-Moro C, et al. 2009. New design strategy for development of specific primer sets for PCR-based detection of Chlorophyceae and Bacillariophyceae in environmental samples. Applied and environmental microbiology. 75:5729-5733. doi: 10.1128/AEM.00509-09.
    Résumé : Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers for Chlorophyceae and Bacillariophyceae by using the ARB software and implementing a virtual PCR program. The results of specificity analysis showed that most of the targeted algal families were identified and nontargeted organisms, such as fungi or ciliates, were excluded. These newly developed PCR primer sets were also able to amplify microalgal rRNA genes from environmental samples with accurate specificity. These tools could be of great interest for studying freshwater microalgal ecology and for developing bioindicators of the health status of aquatic environments.
    Mots-clés : #7, Chlorophyta, computational biology, Computer Simulation, Diatoms, DNA Primers, DNA, Algal, DNA, Ribosomal, Environmental Microbiology, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, RNA, Algal, RNA, Ribosomal, 18S, Sensitivity and Specificity, Sequence Analysis, DNA, Sequence Homology.

  • Valiente-Moro C, et al. 2009. The poultry red mite (Dermanyssus gallinae): a potential vector of pathogenic agents. Experimental & applied acarology. 48:93-104. doi: 10.1007/s10493-009-9248-0.
    Résumé : The poultry red mite, D. gallinae has been involved in the transmission of many pathogenic agents, responsible for serious diseases both in animals and humans. Nowadays, few effective methods are available to control the ectoparasite in poultry farms. Consequently, this is an emerging problem which must be taken into account to maintain good health in commercial egg production. This paper addresses the vector capacity of the ectoparasite with special emphasis on salmonellae, pathogenic agents responsible for many of the most important outbreaks of food-borne diseases worlwide. It has been experimentally shown that D. gallinae could act as a biological vector of S. enteritidis and natural carriage of these bacteria by the mite on poultry premises has also been reported. It was also found that D. gallinae carried other pathogens such as E. coli, Shigella sp., and Staphylococcus, thus increasing the list of pathogenic agents potentially transmitted by the mite.
    Mots-clés : Animals, Arachnid Vectors, Host-Parasite Interactions, Humans, Mites, Poultry, Salmonella enteritidis, Salmonella Infections, Animal.

  • Valiente-Moro C, Thioulouse J, Chauve C, Normand P, Zenner L. 2009. Bacterial taxa associated with the hematophagous mite <i>Dermanyssus gallinae</i> detected by 16S rRNA PCR amplification and TTGE fingerprinting. Research in Microbiology. 160:63–70.
    Mots-clés : #1, #7.


  • Valiente-Moro C, Desloire S, Chauve C, Zenner L. 2007. Detection of Salmonella sp. in Dermanyssus gallinae using an FTA® filter-based polymerase chain reaction. Medical and Veterinary Entomology. 21:148-152. doi: 10.1111/j.1365-2915.2007.00684.x.
    Résumé : AbstractSalmonella spp. bacteria are responsible for some of the most important zoonoses worldwide. Because Dermanyssus gallinae (DeGeer) (Acari: Dermanyssidae) has been recently reported to be an experimental vector of Salmonella Enteritidis, it would be of benefit to evaluate the presence of this bacterium in mites. A molecular detection tool associating a simple filter-based DNA preparation with a specific 16S rDNA Salmonella sp. polymerase chain reaction (PCR) amplification was described. The limit of detection with this method was 2 × 104 bacteria per mite. To adapt this technique for large-scale studies, two sizes of mite pools were tested and a preliminary investigation was carried out on mites from 16 currently or previously contaminated farms. Mites sampled from one farm of each type were positive for Salmonella, suggesting that Dermanyssus could act as a reservoir between flocks. In further investigations, it will be necessary to carry out a large-scale study to assess the role of D. gallinae in the epidemiology of avian salmonellosis.
    Mots-clés : Dermanyssus gallinae, filter paper DNA preparation, PCR detection, Salmonella.

  • Valiente-Moro C, Desloire S, Vernozy-Rozand C, Chauve C, Zenner L. 2007. Comparison of the VIDAS® system, FTA® filter-based PCR and culture on SM ID for detecting Salmonella in Dermanyssus gallinae. Letters in Applied Microbiology. 44:431-436. doi: 10.1111/j.1472-765X.2007.02119.x.
    Résumé : Aims:  To compare different analytical methods for detecting Salmonella in Dermanyssus gallinae. Methods and Results:  The detection limit of three Salmonella detection methods [Vitek immunodiagnostic assay (VIDAS®) Salmonella immuno-concentration/immunoassay, FTA® filter-based PCR, and Salmonella detection and identification medium (SM ID) preceded by a pre-enrichment step] was evaluated by crushing mites in serial dilutions of pure cultures of Salmonella enterica ssp. Enterica serotype Enteritidis. Each method was then compared for its ability to detect Salmonella in artificially contaminated mites. In 105 mites artificially engorged with Salm. Enteritidis-contaminated blood, Salmonella was isolated from 68 samples of the samples cultured on SM ID and tests were positive for Salmonella using FTA filter-based PCR and VIDAS in 77 and 65 samples, respectively. Using SM ID as our reference method, specificities and sensitivities were 97% and 94% and 73% and 98·5% for VIDAS and PCR, respectively. Conclusions:  Each method allowed the detection of Salmonella in contaminated mites and is usable for screening mites. PCR is more sensitive but less specific than VIDAS for detecting Salmonella. Significance and Impact of the Study:  This is the first time that the VIDAS has been used to detect pathogens in vectors. The development of analytical methods for Salmonella detection in mites is a necessary step in the study of the role of D. gallinae as a vector of salmonellae and to check the contamination of D. gallinae in poultry facilities.
    Mots-clés : Dermanyssus gallinae, PCR, Poultry, Salmonella, SM ID, VIDAS®.

  • Valiente-Moro C, et al. 2007. Colonization and organ invasion in chicks experimentally infected with Dermanyssus gallinae contaminated by Salmonella Enteritidis. Avian Pathology. 36:307-311. doi: 10.1080/03079450701460484.
    Résumé : The poultry red mite (Dermanyssus gallinae) is the most important and common ectoparasite of laying hens in Europe. This haematophagous mite has been experimentally demonstrated to be a vector of Salmonella Enteritidis by acquiring bacteria through the blood meal or cuticular contact. We have evaluated another route of infection by orally inoculating chicks with mites previously infected by S. Enteritidis. Two methods of infecting the mites were tested: mites contaminated by cuticular contact or during the blood meal. After the washing of mites with paraformaldehyde, groups of 10 Salmonella-contaminated mites were inoculated individually into 1-day-old chicks. The titre of the inoculum suspension was evaluated by crushing mites and followed by bacteriological counting. It was 3×104 colony-forming units/chick and 2.7×106 colony-forming units/chick, respectively, for cuticular contact and orally mediated contamination of mites. Each bird was found to be positive 12 days post-inoculation. Salmonella colonized the intestinal tracts and invaded the livers and spleens. The caecal content concentration reached a mean level of S. Enteritidis of 8.5×104 most probable number (MPN) Salmonella/g. This experiment demonstrated the ability of mites to orally infect 1-day-old chicks with subsequent colonization and multiplication of Salmonella. Consequently, mites infected by S. Enteritidis constitute potential reservoir hosts of this bacterium, allowing it to persist in the poultry house as a source of infection for newly introduced animals. If contaminated mites are found in poultry facilities, effective red mite control should be performed before new batches are introduced into the facility.


  • Desloire S, Valiente-Moro C, Chauve C, Zenner L. 2006. Comparison of four methods of extracting DNA from D. gallinae (Acari: Dermanyssidae). Veterinary Research. 37:8. doi: 10.1051/vetres:2006031.


  • Valiente-Moro C, Chauve C, Zenner L. 2005. Vectorial role of some dermanyssoid mites (Acari, Mesostigmata, Dermanyssoidea). Parasite. 12:11. doi: 10.1051/parasite/2005122099.

Chapitres d’ouvrages


Communications Orales



  • Minard G, et al. 2014. Exploring the role of bacterial microbiota in invasive capacities of the tiger mosquito Aedes albopictus. In: Tahoe City, California, USA p. .
    Mots-clés : #poster.


  • Raquin V, et al. 2013. Potential role of autophagy during Wolbachia antiviral interference against chikungunya virus in mosquito cells. In: Lyon, France p. .
    Mots-clés : #poster.


  • Minard G. 2012. Phylotypage des communautés bactériennes associées au moustique tigre Aedes albopictus. In: Journées Scientifiques ARC1 p. .
    Mots-clés : #7, #ibio, #poster.