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Accueil > Pages Perso > Patrick Mavingui

Equipe de Recherche Dynamique microbienne et transmission virale

Patrick Mavingui

Directeur de recherche CNRS

Contact :
patrick.mavingui univ-lyon1.fr

Scientific Expertise

Molecular genetics and of bacteria interacting with eukaryotic hosts, mainly dealing with symbioses, genome dynamics, mechanisms of cellular and molecular communication, differential gene expression. Molecular microbial ecology, aiming to determine key determinants that control communication and proliferation of bacteria in the eukaryote hosts (plants, invertebrates, mammals). Characterization of bacterial effectors and host targets, using biochemical, immunological, genetic and fluorescence spectroscopy techniques. Ecology and health, mosquito-borne diseases (arboviroses).

Education

  • 2009 Habilitation, Université de Lyon 1, Symbiosis and Evolution
  • 1992 Ph.D., University of Nancy I, France, Microbiology , Microbial Ecology
  • 1987 Master, Institut National Polytechnique de Lorraine, Nancy, France

Professional Experience

  • Since 2011 Research Director at CNRS, University Lyon 1, France
  • 2002-2010 Chargé de recherches at CNRS, University Lyon 1, France
  • 2001 Assistant researcher, University of Geneva
  • 1994-2001 Researcher, National Autonomous University of Mexico
  • 1993 Post-doctoral Laboratory of Soil Microbiology, INRA, Dijon, France

Administration

  • Director of GDRI “Biodiversité et Développement Durable à Madagascar”
  • Deputy Director of Research Federation FR41 “BioEnvironment University of Lyon 1
  • Head leader of the team « Microbial Dynamics and Viral Transmission »

Referee for Scientific Organisations

  • Head VLIR-UOS–Belgium Own Initiatives
  • ANR, EC2CO, Institut Pasteur
  • Bart Israel

Referee for Journals

More than 10 journals in Ecology, Parasitology, Entomology, Microbiology

Selected Grants

  • ANR blanc 2006 « Endosymbiosis in Arthropods (Endosymbart) » (2007-2009).
  • ANR SEST 2006 « Multipartite interactions virus Chikungunya-Endosymbionts-Moquitoes : Impact on vector competence »
  • UE STREP Food Quality & Safety. Micromaize. (2006-2009)
  • IFB/FRB 2008 « Environnement and risk emergence and reemergence or arboviroses in Madagascar ».
  • ACIP Pasteur 2009 « Does the endosymbiont Wolbachia able to affect dengue and chikungunya transmission ? » (2009-2010)
  • ANR blanc « Immunity and Symbiosis in Arthropods » (2011-2014)

Publications

2017

Article de revue


  • Goubert C, et al. 2017. High-throughput sequencing of transposable element insertions suggests adaptive evolution of the invasive Asian tiger mosquito towards temperate environments. Molecular Ecology. 26:3968-3981. doi: 10.1111/mec.14184.
    Résumé : Invasive species represent unique opportunities to evaluate the role of local adaptation during colonization of new environments. Among these species, the Asian tiger mosquito, Aedes albopictus, is a threatening vector of several human viral diseases, including dengue and chikungunya, and raises concerns about the Zika fever. Its broad presence in both temperate and tropical environments has been considered the reflection of great “ecological plasticity.” However, no study has been conducted to assess the role of adaptive evolution in the ecological success of Ae. albopictus at the molecular level. In the present study, we performed a genomic scan to search for potential signatures of selection leading to local adaptation in one-hundred-forty field-collected mosquitoes from native populations of Vietnam and temperate invasive populations of Europe. High-throughput genotyping of transposable element insertions led to the discovery of more than 120,000 polymorphic loci, which, in their great majority, revealed a virtual absence of structure between the biogeographic areas. Nevertheless, 92 outlier loci showed a high level of differentiation between temperate and tropical populations. The majority of these loci segregate at high insertion frequencies among European populations, indicating that this pattern could have been caused by recent adaptive evolution events in temperate areas. An analysis of the overlapping and neighbouring genes highlighted several candidates, including diapause, lipid and juvenile hormone pathways.
    Mots-clés : #7, Aedes albopictus, genome scan, invasive species, local adaptation, transposable elements.


  • Minard G, et al. 2017. Identification of sympatric cryptic species of Aedes albopictus subgroup in Vietnam: new perspectives in phylosymbiosis of insect vector. Parasites & Vectors. 10:276. doi: 10.1186/s13071-017-2202-9.
    Résumé : The Aedes (Stegomyia) albopictus subgroup includes 11 cryptic species of which Ae. albopictus is the most widely distributed. Its global expansion associated with a documented vector competence for several emerging arboviruses raise obvious concerns in the recently colonized regions. While several studies have provided important insights regarding medical importance of Ae. albopicus, the investigations of the other sibling species are scarce. In Asia, indigenous populations within the Ae. albopictus subgroup can be found in sympatry. In the present study, we aimed to describe and compare molecular, morphological and bacterial symbionts composition among sympatric individuals from the Ae. albopictus subgroup inhabiting a Vietnamese protected area.
    Mots-clés : #7, Asian tiger mosquito, Dysgonomonas, Microbiota.
    Note Note
    <p>Pages 276 in PDF</p>
    Note Note
    Pages 276 in PDF


  • René-Martellet M, et al. 2017. Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona. Parasites & Vectors. 10:416. doi: 10.1186/s13071-017-2352-9.
    Résumé : Ticks of the group Rhipicephalus sanguineus (sensu lato) are distributed worldwide and are major pathogen vectors of both dogs and humans. Previous phylogenetic reconstructions have suggested the existence of two main lineages within this group, “Tropical” and “Temperate”. Symbiotic interactions contribute to vector development, survival, reproduction and competence. The diversity of microbial communities associated with different populations of R. sanguineus (s.l.) remains poorly characterized, however, this knowledge will aid in future studies of hosts-microbiota-pathogen interactions. To gain insight into the bacterial communities associated with R. sanguineus (s.l.) ticks, 40 specimens from France, Senegal and Arizona were analyzed by high-throughput 16S amplicon sequencing. All tick specimens were taxonomically classified using the mitochondrial 12S rDNA gene, which provides sufficient phylogenetic resolution to discriminate different lineages of R. sanguineus.
    Mots-clés : #7, Bacillus, Coxiella, Rhipicephalus sanguineus, Rickettsia, Symbionts.
    Note Note
    Pages 416 in PDF


  • Saucereau Y, et al. 2017. Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon. BMC Genomics. 18:635. doi: 10.1186/s12864-017-3985-y.
    Résumé : Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of Ae. albopictus varies depending on the mosquito population involved and the virus transmitted. Wolbachia infection status in believed to be among key elements that determine viral transmission efficiency. Little is known about the cellular functions mobilized in Ae. albopictus during co-infection by Wolbachia and a given arbovirus. To decipher this tripartite interaction at the molecular level, we performed a proteome analysis in Ae. albopictus C6/36 cells mono-infected by Wolbachia wAlbB strain or Chikungunya virus (CHIKV), and bi-infected.
    Mots-clés : #7, Aedes albopictus, Glycolysis, Metabolism, miRNA, Proteome, Viral inhibition, Wolbachia.
    Note Note
    Pages 635 in PDF


  • Zotzmann S, et al. 2017. Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany. Parasitology Research. 116:1899-1906. doi: 10.1007/s00436-017-5466-2.
    Résumé : Symbiotic bacteria have gained significant attention in recent years. For example, microbiota of some mosquito species seems to influence the development and transmission of pathogens. Furthermore, several attempts using bacteria as a paratransgenetic tool have been made in order to assist the control of mosquito-borne diseases. In this study, we examined the bacterial diversity of wild-caught adult Culex (Cx.) pipiens and laboratory-reared adult Aedes japonicus (Ae. japonicus) in Germany using a culture-independent method. Genomic DNA was extracted from each specimen and submitted to PCR amplification of eubacterial 16S rDNA. After the cloning reaction, 28 bacterial transformants per sample containing the 16S rDNA inserts were selected per each sample for sequencing. The analysed specimens of Cx. pipiens as well as of Ae. japonicus showed a diverse bacterial community including some common bacterial genera. Blast analysis allowed to identify 21 bacterial genera belonging to 2 phyla among the 23 specimens of Cx. pipiens. The 14 analysed Ae. japonicus revealed 11 bacterial genera belonging to 3 phyla. In both mosquito species, identified isolates were mainly Proteobacteria. Only 4 of the bacterial genera were found in both mosquito species, with the most prevalent genera Sphingomonas and Rahnella in Cx. pipiens and in Ae. japonicus respectively. Most of the bacterial genera found in our study have been identified in other mosquito species before. Due to the currently scarce data situation, ongoing examinations on the very abundant bacterial genera or species are strongly required to determine their relevance for the biology and adaptiveness of mosquitoes including pathogen-host relationship.
    Mots-clés : #7.

2016

Article de revue


  • Goubert C, et al. 2016. High-Throughput Sequencing of Transposable Elements Insertions Provides Evidence for Adaptive Evolution of the Invasive Asian Tiger Mosquito Towards Temperate Environments. bioRxiv. doi: 10.1101/049197.
    Résumé : Invasive species represent unique opportunities to evaluate the role of local adaptation during colonization of new environments. Among these, the Asian tiger mosquito, Aedes albopictus, is a threatening vector of several human viral diseases, including dengue, chikungunya and the emerging Zika fevers. Its broad presence in both temperate and tropical environments has sometimes been considered as the reflect of a great "ecological plasticity". However, no study has been conducted to assess the role of adaptive evolution in the ecological success of Ae. albopictus at the molecular level. In the present study we performed a genomic scan to search for potential signatures of selection leading to local adaptation in a hundred of field collected mosquitoes from native populations of Vietnam and temperate invasive populations of Europe. High throughput genotyping of transposable element insertions generated more than 120 000 polymorphic loci, which in their great majority revealed a virtual absence of structure between bio-geographic areas. Nevertheless, 92 outlier loci show a high level of differentiation between temperate and tropical populations. The majority of these loci segregates at high insertion frequencies among European populations, indicating that this pattern could have been caused by recent events of adaptive evolution in temperate areas. Six outliers were located near putative diapause effector genes, suggesting fine tunning of this critical pathway during local adaptation.
    Mots-clés : #7.


  • Moutailler S, et al. 2016. Co-infection of Ticks: The Rule Rather Than the Exception Vinetz, JM. PLOS Neglected Tropical Diseases. 10:e0004539. doi: 10.1371/journal.pntd.0004539.
    Mots-clés : #7.


  • Wilkinson DA, et al. 2016. The bacteriome of bat flies (Nycteribiidae) from the Malagasy region: a community shaped by host ecology, bacterial transmission mode, and host-vector specificity. Applied and Environmental Microbiology. AEM.03505-15. doi: 10.1128/AEM.03505-15.

2015

Article de revue


  • Goubert C, et al. 2015. De novo assembly and annotation of the Asian tiger mosquito (Aedes albopictus) repeatome with dnaPipeTE from raw genomic reads and comparative analysis with the yellow fever mosquito (Aedes aegypti). Genome Biology and Evolution. doi: 10.1093/gbe/evv050.
    Mots-clés : #7.


  • Minard G, et al. 2015. French invasive Asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to Vietnamese autochthonous relatives. Microbial Symbioses. 6:970. doi: 10.3389/fmicb.2015.00970.
    Résumé : The Asian tiger mosquito Aedes albopictus is one of the most significant pathogen vectors of the 21st century. Originating from Asia, it has invaded a wide range of eco-climatic regions worldwide. The insect-associated microbiota is now recognized to play a significant role in host biology. While genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. To address this subject, we compared four autochthonous Ae. albopictus populations in Vietnam, the native area of Ae. albopictus, and three populations recently introduced to Metropolitan France, with the aim of documenting whether these populations display differences in host genotype and bacterial microbiota. Population-level genetic diversity (microsatellite markers and COI haplotype) and bacterial diversity (16S rDNA metabarcoding) were compared between field-caught mosquitoes. Bacterial microbiota from the whole insect bodies were largely dominated by Wolbachia pipientis. Targeted analysis of the gut microbiota revealed a greater bacterial diversity in which a fraction was common between French and Vietnamese populations. The genus Dysgonomonas was the most prevalent and abundant across all studied populations. Overall genetic diversities of both hosts and bacterial microbiota were significantly reduced in recently established populations of France compared to the autochthonous populations of Vietnam. These results open up many important avenues of investigation in order to link the process of geographical invasion to shifts in commensal and symbiotic microbiome communities, as such shifts may have dramatic impacts on the biology and/or vector competence of invading hematophagous insects.
    Mots-clés : #7, Aedes albopictus, Dysgonomonas, holobiont, Microbiota, Phylogeography, Wolbachia.


  • Raquin V, et al. 2015. Native Wolbachia from Aedes albopictus Blocks Chikungunya Virus Infection In Cellulo Bourtzis, K. PLOS ONE. 10:e0125066. doi: 10.1371/journal.pone.0125066.
    Mots-clés : #7.


  • René-Martellet M, et al. 2015. Update on epidemiology of canine babesiosis in Southern France. BMC Veterinary Research. 11. doi: 10.1186/s12917-015-0525-3.
    Mots-clés : #7.
Chapitre de livre

  • Wisniewski-Dyé F, et al. 2015. Core and Accessory Genomes of the Diazotroph Azospirillum. In: Biological Nitrogen Fixation, 2 Volume Set. p. 253. http://books.google.fr/books?hl=fr&lr=&id=TR3yCQAAQBAJ&oi=fnd&pg=PA253&dq=Core+and+Accessory+Genomes+of+the+Diazotroph+Azospirillum&ots=E7vL8QMeo2&sig=36tsUglxpPtMgpPO21Y95flMDvc.
    Mots-clés : #3, #7.

2014

Article de revue


  • Minard G, et al. 2014. Pyrosequencing 16S rRNA genes of bacteria associated with wild tiger mosquito Aedes albopictus: a pilot study. Frontiers in Cellular and Infection Microbiology. 4. doi: 10.3389/fcimb.2014.00059.
    Résumé : The Asian tiger mosquito Aedes (Stegomya) albopictus is an invasive species that has spread across the world in the last two decades, showing a great capacity to adapt to contrasting climates and environments. While demonstrated in many insects, the contribution of bacterial symbionts in Aedes ecology is a challenging aspect that needs to be investigated. Also some bacterial species have already been identified in Ae. albopictus using classical methods, but a more accurate survey of mosquito-associated bacterial diversity is needed to decipher the potential biological functions of bacterial symbionts in mediating or constraining insect adaptation. We surveyed the bacteria associated with field populations of Ae. albopictus from Madagascar by pyrosequencing 16S rRNA gene amplicons. Different aspects of amplicon preparation and sequencing depth were tested to optimize the breadth of bacterial diversity identified. The results revealed that all mosquitoes collected from different sites have a bacterial microbiota dominated by a single taxon, Wolbachia pipientis, which accounted for about 99% of all 92,615 sequences obtained. As Ae. albopictus is known to harbor two Wolbachia strains (wAlbA and wAlbB), a quantitative PCR was used to estimate the relative densities, (i.e., the bacteria-to-host gene ratios) of each strains in individual mosquitoes. Relative densities were between 6.25 × 100.01 and 5.47 × 100.1 for wAlbA and between 2.03 × 100.1 and 1.4 × 101 for wAlbB. Apart from Wolbachia, a total of 31 bacterial taxa were identified at the genus level using different method variations. Diversity index values were low and probably underestimated the true diversity due to the high abundance of Wolbachia sequences vastly outnumbering sequences from other taxa. Further studies should implement alternative strategies to specifically discard from analysis any sequences from Wolbachia, the dominant endosymbiotic bacterium in Ae. albopictus from this area.
    Mots-clés : #7, #ibio.
Article de colloque
  • Minard G, et al. 2014. Exploring the role of bacterial microbiota in invasive capacities of the tiger mosquito Aedes albopictus. In: Tahoe City, California, USA p. .
    Mots-clés : #poster.
  • Minard G. 2014. Evidence for a core bacterial microbiota among geographically and genetically different populations of the tiger mosquito Aedes albopictus. In: Lyon p. .
  • Valiente Moro C, et al. 2014. Are Wolbachia and microbiota linked to the genetic structure of invasive and endemic populations of Aedes albopictus? In: Igls, Austria p. .

2013

Article de revue


  • Bourtzis K, et al. 2013. Harnessing mosquito–Wolbachia symbiosis for vector and disease control. Acta Tropica. doi: 10.1016/j.actatropica.2013.11.004.
    Résumé : Abstract Mosquito species, members of the genera Aedes, Anopheles and Culex, are the major vectors of human pathogens including protozoa (Plasmodium sp.), filariae and of a variety of viruses (causing dengue, chikungunya, yellow fever, West Nile). There is lack of efficient methods and tools to treat many of the diseases caused by these major human pathogens, since no efficient vaccines or drugs are available; even in malaria where insecticide use and drug therapies have reduced incidence, 219 million cases still occurred in 2010. Therefore efforts are currently focused on the control of vector populations. Insecticides alone are insufficient to control mosquito populations since reduced susceptibility and even resistance is being observed more and more frequently. There is also increased concern about the toxic effects of insecticides on non-target (even beneficial) insect populations, on humans and the environment. During recent years, the role of symbionts in the biology, ecology and evolution of insect species has been well-documented and has led to suggestions that they could potentially be used as tools to control pests and therefore diseases. Wolbachia is perhaps the most renowned insect symbiont, mainly due to its ability to manipulate insect reproduction and to interfere with major human pathogens thus providing new avenues for pest control. We herein present recent achievements in the field of mosquito–Wolbachia symbiosis with an emphasis on Aedes albopictus. We also discuss how Wolbachia symbiosis can be harnessed for vector control as well as the potential to combine the sterile insect technique and Wolbachia-based approaches for the enhancement of population suppression programs.
    Mots-clés : #7, Aedes albopictus, Mosquitoes, Sterile insect technique, Vector control, Wolbachia.


  • Minard G, Mavingui P, Valiente Moro C. 2013. Diversity and function of bacterial microbiota in the mosquito holobiont. Parasites & Vectors. 6:146. doi: 10.1186/1756-3305-6-146.
    Résumé : Mosquitoes (Diptera: Culicidae) have been shown to host diverse bacterial communities that vary depending on the sex of the mosquito, the developmental stage, and ecological factors. Some studies have suggested a potential role of microbiota in the nutritional, developmental and reproductive biology of mosquitoes. Here, we present a review of the diversity and functions of mosquito-associated bacteria across multiple variation factors, emphasizing recent findings. Mosquito microbiota is considered in the context of possible extended phenotypes conferred on the insect hosts that allow niche diversification and rapid adaptive evolution in other insects. These kinds of observations have prompted the recent development of new mosquito control methods based on the use of symbiotically-modified mosquitoes to interfere with pathogen transmission or reduce the host life span and reproduction. New opportunities for exploiting bacterial function for vector control are highlighted.
    Mots-clés : #7, bacterial community, Extended phenotype, Microbiome, Mosquito, Symbiont.

  • Minard G, et al. 2013. Prevalence, genomic and metabolic profiles of <i>Acinetobacter</i> and <i>Asaia</i> associated with field-caught <i>Aedes albopictus</i> from Madagascar. FEMS Microbiology Ecology. http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2012.01455.x/full.
    Mots-clés : #7.


  • Valiente Moro C, Tran FH, Raharimalala FN, Ravelonandro P, Mavingui P. 2013. Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus. BMC Microbiology. 13:70. doi: 10.1186/1471-2180-13-70.
    Résumé : The microbiota has been shown to play an important role in the biology of insects. In recent decades, significant efforts have been made to better understand the diversity of symbiotic bacteria associated with mosquitoes and assess their influence on pathogen transmission. Here, we report the bacterial composition found in field-caught Aedes albopictus populations by using culture-dependent methods.
    Mots-clés : #7.
Article de colloque
  • Raquin V, et al. 2013. Potential role of autophagy during Wolbachia antiviral interference against chikungunya virus in mosquito cells. In: Lyon, France p. .
    Mots-clés : #poster.

2012

Article de revue

  • Acosta-Cruz E, et al. 2012. Insights into the 1.59-Mbp largest plasmid of <i>Azospirillum brasilense</i> CBG497. Archives of microbiology. 1–12. http://www.springerlink.com/index/J2565133L2510914.pdf.
    Mots-clés : #3, #7.

  • Kremer N, et al. 2012. Influence of <i>Wolbachia</i> on host gene expression in an obligatory symbiosis. BMC microbiology. 12:S7. http://www.biomedcentral.com/1471-2180/12/S1/S7/.
    Mots-clés : #7.


  • Mavingui P, et al. 2012. Whole-Genome Sequence of <i>Wolbachia</i> Strain wAlbB, an Endosymbiont of Tiger Mosquito Vector <i>Aedes albopictus</i>. Journal of Bacteriology. 194:1840-1840. doi: 10.1128/JB.00036-12.
    Résumé : Although bacteria of the genus Wolbachia induced significant extended phenotypes to infected hosts, most molecular mechanisms involved are still unknown. To gain insight into the bacterial genetic determinants, we sequenced the whole genome of Wolbachia wAlbB strain, a commensal obligate intracellular of the tiger mosquito Aedes albopictus.
    Mots-clés : #3, #7.


  • Mousson L, et al. 2012. The Native Wolbachia Symbionts Limit Transmission of Dengue Virus in Aedes albopictus. PLoS Negl Trop Dis. 6:e1989. doi: 10.1371/journal.pntd.0001989.
    Résumé : Aedes albopictus is an invasive species that is expanding its natural range of geographic distribution. While it was previously considered a secondary vector of different arboviruses, this mosquito species is involved in the most recent outbreaks of chikungunya but contributes weakly to dengue outbreaks. Ae. albopictus naturally carries two strains of the bacterium Wolbachia, wAlbA and wAlbB. Present in 20% of insect species, Wolbachia is an obligate intracellular symbiont mainly transmitted through females. When inoculated into some mosquito hosts, Wolbachia is able to shorten the adult life span and to block arbovirus transmission. We have previously shown that Wolbachia is not capable of limiting chikungunya replication in the mosquito vector. In this study, we show that the native Wolbachia is able to limit dengue transmission by restricting the delivery of infectious viral particles from the mosquito saliva when biting. Therefore, our results might explain the low vector competence of Ae. albopictus for dengue, and thus its weak contribution as an epidemic dengue vector.
    Mots-clés : #7.


  • Raharimalala F, et al. 2012. Biogeography of the two major arbovirus mosquito vectors, Aedes aegypti and Aedes albopictus (Diptera, Culicidae), in Madagascar. Parasites & Vectors. 5:56. doi: 10.1186/1756-3305-5-56.
    Résumé : In the past ten years, the Indian Ocean region has been the theatre of severe epidemics of chikungunya and dengue. These outbreaks coincided with a high increase in populations of Aedes albopictus that outcompete its sister taxon Aedes aegypti in most islands sampled. The objective of this work was to update the entomological survey of the two Aedes species in the island of Madagascar which has to face these arboviroses.
    Mots-clés : #7.

  • Raquin V, et al. 2012. Detection of dengue group viruses by fluorescence in situ hybridization. Parasites & vectors. 5:243. doi: 10.1186/1756-3305-5-243.
    Résumé : ABSTRACT: BACKGROUND: Dengue fever (DF) and dengue hemorrhagic fever (DHF) represent a global challenge in public health. It is estimated that 50 to 100 million infections occur each year causing approximately 20,000 deaths that are usually linked to severe cases like DHF and dengue shock syndrome. The causative agent of DF is dengue virus (genus Flavivirus) that comprises four distinct serotypes (DENV-1 to DENV-4). Fluorescence in situ hybridization (FISH) has been used successfully to detect pathogenic agents, but has not been implemented in detecting DENV. To improve our understanding of DENV infection and dissemination in host tissues, we designed specific probes to detect DENV in FISH assays. METHODS: Oligonucleotide probes were designed to hybridize with RNA from the broadest range of DENV isolates belonging to the four serotypes, but not to the closest Flavivirus genomes. Three probes that fit the criteria defined for FISH experiments were selected, targeting both coding and non-coding regions of the DENV genome. These probes were tested in FISH assays against the dengue vector Aedes albopictus (Diptera: Culicidae). The FISH experiments were led in vitro using the C6/36 cell line, and in vivo against dissected salivary glands, with epifluorescence and confocal microscopy. RESULTS: The three 60-nt oligonucleotides probes DENV-Probe A, B and C cover a broad range of DENV isolates from the four serotypes. When the three probes were used together, specific fluorescent signals were observed in C6/36 infected with each DENV serotypes. No signal was detected in either cells infected with close Flavivirus members West Nile virus or yellow fever virus. The same protocol was used on salivary glands of Ae. albopictus fed with a DENV-2 infectious blood-meal which showed positive signals in the lateral lobes of infected samples, with no significant signal in uninfected mosquitoes. CONCLUSION: Based on the FISH technique, we propose a way to design and use oligonucleotide probes to detect arboviruses. Results showed that this method was successfully implemented to specifically detect DENV in a mosquito cell line, as well as in mosquito salivary glands for the DENV-2 serotype. In addition, we emphasize that FISH could be an alternative method to detect arboviruses in host tissues, also offering to circumvent the discontinuity of antibodies used in immunofluorescent assays.
    Mots-clés : #7.

  • René M, et al. 2012. First evidence and molecular characterization of<i> Babesia vogeli</i> in naturally infected dogs and<i> Rhipicephalus sanguineus</i> ticks in southern France. Veterinary parasitology. http://www.sciencedirect.com/science/article/pii/S0304401712000490.
    Mots-clés : #7.


  • Wisniewski-Dyé F, et al. 2012. Genome Sequence of <i>Azospirillum brasilense</i> CBG497 and Comparative Analyses of <i>Azospirillum</i> Core and Accessory Genomes provide Insight into Niche Adaptation. Genes. 3:576-602. doi: 10.3390/genes3040576.
    Mots-clés : #3, #7, #ibio.


  • Zouache K, Michelland RJ, Failloux AB, Grundmann G, Mavingui P. 2012. Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector. Molecular Ecology. 21:2297–2309. doi: 10.1111/j.1365-294X.2012.05526.x.
    Résumé : Mosquitoes transmit numerous arboviruses including dengue and chikungunya virus (CHIKV). In recent years, mosquito species Aedes albopictus has expanded in the Indian Ocean region and was the principal vector of chikungunya outbreaks in La Reunion and neighbouring islands in 2005 and 2006. Vector-associated bacteria have recently been found to interact with transmitted pathogens. For instance, Wolbachia modulates the replication of viruses or parasites. However, there has been no systematic evaluation of the diversity of the entire bacterial populations within mosquito individuals particularly in relation to virus invasion. Here, we investigated the effect of CHIKV infection on the whole bacterial community of Ae. albopictus. Taxonomic microarrays and quantitative PCR showed that members of Alpha- and Gammaproteobacteria phyla, as well as Bacteroidetes, responded to CHIKV infection. The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection, whereas the abundance of known insect endosymbionts like Wolbachia and Blattabacterium decreased. Our results clearly link the pathogen propagation with changes in the dynamics of the bacterial community, suggesting that cooperation or competition occurs within the host, which may in turn affect the mosquito traits like vector competence.
    Mots-clés : #3, #7, Aedes albopictus, bacterial community, chikungunya, microarray, quantitative PCR, Wolbachia.
Article de colloque
  • Minard G, et al. 2012. Acinetobacter is more prevalent than Asaia in field populations of the mosquito vector Aedes albopictus with isolates showing diverse genetic rearrangements and metabolic profiles. In: Oléron, France p. .
  • Minard G. 2012. Phylotypage des communautés bactériennes associées au moustique tigre Aedes albopictus. In: Journées Scientifiques ARC1 p. .
    Mots-clés : #7, #ibio, #poster.

2011

Article de revue

  • Berge O, Mavingui P, Heulin T. 2011. Exploring Diversity of Cultivable Aerobic Endospore-forming Bacteria: From Pasteurization to Procedures Without Heat-Shock Selection. Endospore-forming Soil Bacteria. 73–88. http://www.springerlink.com/index/K45M58536478528U.pdf.
    Mots-clés : #7.

  • Vavre F, Mavingui P. 2011. Endosymbionts of arthropods and nematodes: allies to fight infectious diseases? Médecine sciences: M/S. 27:953-958. doi: 10.1051/medsci/20112711010.
    Résumé : Arthropods and nematodes are important protagonists in human health because either they act as vectors of pathogens (bacteria, protozoa, viruses or fungus), or are themselves parasites. Fighting infectious diseases is based essentially on vaccination (prevention) or chemotherapeutic (curative) approaches in human, but one can envisage as an alternative to reduce the number of vectors or limit their ability to spread pathogens. Such strategies controlling dissemination will undoubtedly benefit from the knowledge accumulated by recent works on powerful mechanisms developed by symbiotic insect bacteria such as Wolbachia to popagate in arthropods and nematods. This review summarizes these recent data, and indicate how these mechanisms can be manipulated to reduce the dissemination of insect vectors propagating human diseases.
    Mots-clés : #7, Animals, Arthropods, Bacteria, Bacterial Physiological Phenomena, Communicable Diseases, Host-Parasite Interactions, Humans, Models, Biological, Molecular Targeted Therapy, Nematoda, symbiosis.


  • Wisniewski-Dyé F, et al. 2011. Azospirillum Genomes Reveal Transition of Bacteria from Aquatic to Terrestrial Environments. PLoS Genet. 7:e1002430. doi: 10.1371/journal.pgen.1002430.
    Résumé : Genome sequencing and analysis of plant-associated beneficial soil bacteria Azospirillum spp. reveals that these organisms transitioned from aquatic to terrestrial environments significantly later than the suggested major Precambrian divergence of aquatic and terrestrial bacteria. Separation of Azospirillum from their close aquatic relatives coincided with the emergence of vascular plants on land. Nearly half of the Azospirillum genome has been acquired horizontally, from distantly related terrestrial bacteria. The majority of horizontally acquired genes encode functions that are critical for adaptation to the rhizosphere and interaction with host plants.
    Mots-clés : #1, #3, #7.

  • Zouache K, et al. 2011. Bacterial diversity of field-caught mosquitoes, <i>Aedes albopictus</i> and <i>Aedes aegypti</i>, from different geographic regions of Madagascar. FEMS microbiology ecology. 75:377–389. http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2010.01012.x/full.
    Mots-clés : #7.
Article de colloque
  • Minard G, et al. 2011. Prevalence of Acinetobacter and Asaia in natural populations of the mosquito vector Aedes albopictus and evidence of high diversity in genomic architecture and phenotype microarray of mosquito-associated Acinetobacter isolates. In: Muséum National d’Histoire Naturelle, Paris, France p. .
  • Minard G. 2011. Prévalence, architecture génomique et profilage métabolique des bactéries cultivables dans les populations naturelles d’Aedes albopictus à Madagascar : exemple des bactéries Acinetobacter et Asaia. In: Antananarivo, Madagascar p. .
  • Valiente Moro C. 2011. L’exploitation des bactéries symbiotiques associées aux moustiques vecteurs: une alternative pour le développement de nouvelles stratégies de contrôle ? In: Strasbourg, France p. .
Chapitre de livre

  • Moënne-Loccoz Y, Mavingui P, Combes C, Normand P, Steinberg C. 2011. Micro-organismes et interactions biotiques. In: Ecologie microbienne: Microbiologie des milieux naturels et anthropisés. Publications de l'Université de Pau et des Pays de l'Adour p. .
    Mots-clés : #1, #3, #7.

2010

Article de revue

  • Couillerot O, et al. 2010. Assessment of SCAR markers to design real-time PCR primers for rhizosphere quantification of <i>Azospirillum brasilense</i> phytostimulatory inoculants of maize. Journal of applied microbiology. 109:528–538. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04673.x/full.
    Mots-clés : #3, #7.

  • Mousson L, et al. 2010. <i>Wolbachia</i> modulates Chikungunya replication in <i>Aedes albopictus</i>. Molecular ecology. 19:1953–1964. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-294X.2010.04606.x/full.
    Mots-clés : #7, Aedes, Alphavirus Infections, Animals, Chikungunya virus, DNA, Bacterial, Female, Oviposition, RNA, Viral, Salivary Glands, symbiosis, Virus Replication, Wolbachia.

  • Poté J, Bravo AG, Mavingui P, Ariztegui D, Wildi W. 2010. Evaluation of quantitative recovery of bacterial cells and DNA from different lake sediments by Nycodenz density gradient centrifugation. Ecological Indicators. 10:234–240. http://www.sciencedirect.com/science/article/pii/S1470160X09000946.
    Mots-clés : #7.

  • Voronin D, Tran Van V, Potier P, Mavingui P. 2010. Transinfection and growth discrepancy of <i>Drosophila Wolbachia</i> strain wMel in cell lines of the mosquito <i>Aedes albopictus</i>. Journal of applied microbiology. 108:2133–2141. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2009.04621.x/full.
    Mots-clés : #7.

2009

Article de revue

  • Kremer N, et al. 2009. <i>Wolbachia</i> interferes with ferritin expression and iron metabolism in insects. PLoS pathogens. 5:e1000630. http://dx.plos.org/10.1371/journal.ppat.1000630.
    Mots-clés : #7.

  • Mavingui P. 2009. The Megaplasmid pNGR234a of <i>Rhizobium</i> sp. Strain NGR234. Microbial Megaplasmids. 119–132. http://www.springerlink.com/index/674280526V614816.pdf.
    Mots-clés : #7.

  • Poté J, et al. 2009. Extracellular plant DNA in Geneva groundwater and traditional artesian drinking water fountains. Chemosphere. 75:498-504. doi: 10.1016/j.chemosphere.2008.12.048.
    Résumé : DNA, as the signature of life, has been extensively studied in a wide range of environments. While DNA analysis has become central to work on natural gene exchange, forensic analyses, soil bioremediation, genetically modified organisms, exobiology, and palaeontology, fundamental questions about DNA resistance to degradation remain. This paper investigated on the presence of plant DNA in groundwater and artesian fountain (groundwater-fed) samples, which relates to the movement and persistence of DNA in the environment. The study was performed in the groundwater and in the fountains, which are considered as a traditional artesian drinking water in Geneva Champagne Basin. DNA from water samples was extracted, analysed and quantified. Plant gene sequences were detected using PCR amplification based on 18S rRNA gene primers specific for eukaryotes. Physicochemical parameters of water samples including temperature, pH, conductivity, organic matter, dissolved organic carbon (DOC) and total organic carbon (TOC) were measured throughout the study. The results revealed that important quantities of plant DNA can be found in the groundwater. PCR amplification based on 18S rDNA, cloning, RFLP analysis and sequencing demonstrated the presence of plant DNA including Vitis rupestris, Vitis berlandieri, Polygonum sp. Soltis, Boopis graminea, and Sinapis alba in the water samples. Our observations support the notion of plant DNA release, long-term persistence and movement in the unsaturated medium as well as in groundwater aquifers.
    Mots-clés : #7, DNA, Plant, Fresh Water, Phylogeny, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 18S, Water, Water Supply.

  • Zouache K, Voronin D, Tran Van V, Mavingui P. 2009. Composition of bacterial communities associated with natural and laboratory populations of Asobara tabida infected with Wolbachia. Applied and environmental microbiology. 75:3755-3764. doi: 10.1128/AEM.02964-08.
    Résumé : Asobara tabida wasps are fly endoparasitoids that naturally harbor three Wolbachia strains, which induce cytoplasmic incompatibility and control oogenesis. To investigate whether other bacteria play a role in wasp biology, we surveyed the bacterial communities of wild A. tabida populations originating from different regions of France and of laboratory colonies using PCR-denaturing gradient gel electrophoresis and culture methods. Proteobacteria and Firmicutes were found to be the main phyla represented in these populations. Among these were several cultured and uncultured representatives of the genera Acetobacter, Acidomonas, Bacillus, Brevibacillus, Duganella, Herbaspirillum, Pseudomonas, Staphylococcus, and Streptococcus. In addition to Wolbachia, wild individuals harbored Rickettsia, which tended to be lost when insects were reared in the laboratory. The antibiotic treatment used to generate wasp sublines singly infected with Wolbachia also affected the overall bacterial composition, with most fingerprint sequences being characteristic of the family Enterobacteriaceae. We also screened for potentially heritable endosymbionts by PCR and fluorescence in situ hybridization in stable laboratory lines, with only Wolbachia being consistently found in wasp ovaries.
    Mots-clés : Animals, Bacteria, Biodiversity, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial, DNA, Ribosomal, Electrophoresis, Polyacrylamide Gel, France, Gram-Negative Bacterial Infections, Hymenoptera, Molecular Sequence Data, Nucleic Acid Denaturation, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid.

  • Zouache K, et al. 2009. Persistent <i>Wolbachia</i> and cultivable bacteria infection in the reproductive and somatic tissues of the mosquito vector <i>Aedes albopictus</i>. PloS one. 4:e6388. http://dx.plos.org/10.1371/journal.pone.0006388.
    Mots-clés : #7.

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