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Accueil > Productions scientifiques > Autres ... > Prevalence, genomic and metabolic profiles of Acinetobacter and Asaia associated with field-caught Aedes albopictus from Madagascar

Minard, Guillaume ; Tran, Florence Hélène ; Raharimalala, Fara Nantenaina ; Hellard, Eléonore ; Ravelonandro, Pierre ; Mavingui, Patrick ; Moro, Claire Valiente

Prevalence, genomic and metabolic profiles of Acinetobacter and Asaia associated with field-caught Aedes albopictus from Madagascar

FEMS Microbiology Ecology

The presence of cultivable bacteria Acinetobacter and Asaia was recently demonstrated in the mosquito vector Aedes albopictus. However it is not known how prevalent these bacteria are in field populations. Here, the presence of these bacteria in Ae. albopictus populations from Madagascar was diagnosed by amplification of 16S rRNA gene fragments. Both genera were detected at relatively high frequencies, 46% for Asaia and 74% for Acinetobacter. The prevalence of Acinetobacter correlated significantly with mosquito gender, and the prevalence of Asaia with the interaction between mosquito gender and the sampling site. For each bacterial genus, more male than female mosquitoes were infected. Using pulse field gel electrophoresis, no significant difference in genome size was found between Acinetobacter isolates from mosquitoes compared to free-living Acinetobacter. However, a great diversity was observed in plasmid numbers (from 1 to 12) and sizes (from less than 8 to 690 kb). Isolates utilized fewer substrates than free-living isolates, but some substrates known as blood or plant components were specifically utilized by mosquito isolates. Therefore it is likely that a specific subpopulation of Acinetobacter is selected by Ae. albopictus. Overall, this study emphasizes the need to gain a global view on the bacterial partners in mosquito vectors.© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. Allrights reserved

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