Langues du site :

Partenaires

CNRS
UCBL
INRA
ENVL
FR41
IDEX




Rechercher

Sur ce site


Accueil > Productions scientifiques > Autres ... > Evaluation of the Andromas MALDI-TOF MS System for Identification of Aerobically Growing Gram-Positive Bacilli

Farfour, E. ; Leto, J. ; Barritault, M. ; Barberis, C. ; Meyer, J. ; Dauphin, B. ; Guern, A.-S. Le ; Leflèche, A. ; Badell, E. ; Guiso, N. ; Leclercq, A. ; Monnier, A. Le ; Lecuit, M. ; Rodriguez-Nava, V. ; Bergeron, E. ; Raymond, J. ; Vimont, S. ; Bille, E. ; Carbonnelle, E. ; Guet-Revillet, H. ; Lécuyer, H. ; Beretti, J.-L. ; Vay, C. ; Berche, P. ; Ferroni, A. ; Nassif, X. ; Join-Lambert, O.

Evaluation of the Andromas MALDI-TOF MS System for Identification of Aerobically Growing Gram-Positive Bacilli

Journal of Clinical Microbiology

Matrix-associated laser desorption time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. Previous reports using the Biotyper system suggested that this technique requires a preliminary extraction step to identify Gram Positive rods (GPRs), a technical issue that may limit the routine use of this technique to identify pathogenic GPRs in the clinical setting. We tested the accuracy of the MALDI-TOF MS Andromas strategy to identify a set of 659 GPR isolates representing 16 bacterial genera and 73 species by the direct colony method. This bacterial collection included 40 C. diphtheriae, 13 C. pseudotuberculosis, 19 C. ulcerans and 270 other Corynebacterium isolates, 32 L. monocytogenes and 24 other Listeria isolates, 46 Nocardia 75 Actinomyces, 18 Actinobaculum, 11 Propionibacterium acnes 18 Propionibacterium avidum, 30 Lactobacillus, 21 Bacillus, 2 Rhodococcus equi, 2 Erysipelothrix rhusiopathiae, and 38 other GPR isolates, all identified by reference techniques.
98.5% and 1.2% of non-Listeria GPR isolates were identified to the species or genus level respectively. Except for L. grayi isolates that were identified to the species level, all other Listeria isolates were identified to the genus level because of highly similar spectra. These data demonstrate that rapid identification of pathogenic GPRs can be obtained without extraction step by MALDI-TOF mass spectrometry.

Dans la même rubrique :