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Accueil > Productions scientifiques > Autres ... > Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE) : Site characterization and on-site bioremediation

Fayolle-Guichard, Françoise ; Durand, Jonathan ; Cheucle, Mathilde ; Rosell, Mònica ; Michelland, Rory Julien ; Tracol, Jean-Philippe ; Roux, Françoise Le ; Grundman, Geneviève ; Atteia, Olivier ; Richnow, Hans H. ; Dumestre, Alain ; Benoit, Yves

Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE) : Site characterization and on-site bioremediation

Journal of Hazardous Materials

Ethyl tert-butyl ether (ETBE) was detected at high concentration (300 mg L−1) in the groundwater below a gas-station. No significant carbon neither hydrogen isotopic fractionation of ETBE was detected along the plume. ETBE and BTEX biodegradation capacities of the indigenous microflora Pz1-ETBE and of a culture (MC-IFP) composed of Rhodococcus wratislaviensis IFP 2016, Rhodococcus aetherivorans IFP 2017 and Aquincola tertiaricarbonis IFP 2003 showed that ETBE and BTEX degradation rates were in the same range (ETBE : 0.91 and 0.83 mg L−1 h−1 and BTEX : 0.64 and 0.82 mg L−1 h−1, respectively) but tert-butanol (TBA) accumulated transiently at a high level using Pz1-ETBE (74 mg L−1). An on-site pilot plant (2 m3) filled with polluted groundwater and inoculated by MC-IFP, successfully degraded four successive additions of ETBE and gasoline. However, an insignificant ETBE isotopic fractionation was also accompanying this decrease which suggested the involvement of low fractionating-strains using EthB enzymes, but required of additional proofs. The ethB gene encoding a cytochrome P450 involved in ETBE biodegradation (present in R. aetherivorans IFP 2017) was monitored by quantitative real-time polymerase chain reaction (q-PCR) on DNA extracted from water sampled in the pilot plant which yield up to 5 Ã— 106 copies of ethB gene per L−1.

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